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Analysis of ERBB2 Polyubiquitin Chain Topology via MRM Assay

This supplementary figure illustrates the MRM assay designed to assess the relative changes in the topology of ERBB2 polyubiquitin chains. It includes a comprehensive list of peptide sequences and their corresponding m/z values used in the assay. All seven possible ubiquitin linkages were measured, alongside three tryptic peptides from ERBB2 (with residue numbers noted) employed for normalizing relative ubiquitin levels across different samples.

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Analysis of ERBB2 Polyubiquitin Chain Topology via MRM Assay

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  1. Supplementary Figure 1. MRM assay to asses the relative changes in ERBB2 polyubiquitin chain topology. The peptide sequences and m/z values for MRM assays. All 7 possible ubiquitin linkages were measured as well as three tryptic peptides from ErbB2 (residue numbers in brackets) that were used to normalize the relative ubiquitin levels between samples. MRM Ions Q1 Q3 Peptide Sequence Peptide Name

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