'Mrna' diaporamas de présentation

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Repressilator Presentation contents:

Repressilator Presentation contents:. The idea. Experimental overview. The first attemp. The mathematical model. Determination of the appropiate parameters. Experimental setting in more detail. Tunning out to the correct parameters.

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RNA maturation transport & localization

RNA maturation transport & localization. RNA processing: brief overview. RNA export to the cytoplasm: model systems. RNA degradation. Links between RNA processing, transport, degradation. RNA localization in the cytoplasm. All eukaryotic mRNAs are processed. Evidence for checkpoints?.

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Step 2: Translation

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PTRM Class 2008 Dan Wang 9-30-2008

PTRM Class 2008 Dan Wang 9-30-2008. Inefficient mRNA/mRNP reactions are challenged by nuclear surveillance at various points in mRNP biogenesis. Saguez, Olesen & Jensen, Curr Opin in Cell Biol, 2005. Co-transcriptional model for the recruitment of mRNA export factors in yeast.

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Warm up

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mRNA secondary structure optimization using a correlated stem-loop prediction

mRNA secondary structure optimization using a correlated stem-loop prediction. Nucleic Acids Res. 2013 Apr 1;41(6):e73. doi : 10.1093/ nar /gks1473. Epub 2013 Jan 15. Presented by WONG Pak Kan. Agenda. Background Motivation Methodology Results Discussion.

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Paper presentation Lucas Kemper 06.12.2010

Paper presentation Lucas Kemper 06.12.2010. Introduction. microRNA. transport of pre-miRNA by Exportin 5. cleavage by Drosha in the nucleus. cleavage by Dicer in the cytoplasm. formation of the miR NA- i nduced s ilencing c omplex. inducible expression of pri-miRNA with

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Organization of the NPC

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The RNA-Binding Protein KSRP Promotes Decay of

The RNA-Binding Protein KSRP Promotes Decay of b -Catenin mRNA and Is Inactivated by PI3K-AKT Signaling. Roberto Gherzi et al. PLoS Biol . (2006). Decay of ARE-containing mRNA.

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OPM in a nutshell: Bread Baking and Eating example

Modeling the involvement of Rpb4/7 in the mRNA biogenesis and degradation with Object-Process Methodology Dov Dori and Mordechai Choder Technion - Israel Institute of Technology August 2005.

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Central dogma of genetics

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TF + S

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Posttranscriptional Modification of DNA

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0 . 5

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??????????????????????? fim ?? mRNA ???????? mRNA ??????

??????????????????????? fim ?? mRNA ???????? mRNA ??????.

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Dhamija et al.,Supplemental Data

Dhamija et al.,Supplemental Data. Figure S1. A. luciferase coding sequence. mRNA:. Luc. Luc-I ? B ? -3'UTR. Luc-I ? B ? -TSE. B. 1.00. 0.75. luciferase/RNA (fold change). 0.50. 0.25. 0.00. -. -. stimulation:. IL-1. IL-17. TNF. Luc-I ? B ? -3'UTR. mRNA:. Luc. C. 8. 6.

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Dataset One

Dataset One. Dataset Two. Wild type. rhd2-1. Genotype. wer/myb23. cpc/try. Cross section. Transverse-section. RHDZ. Protoplasting + FACS. RNA extraction. Total RNA. Total RNA. mRNA extraction. cRNA probe. cRNA probe. Hybridisation. mRNA. mRNA. ATH1 Microarray.

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