DNA Sequencing. From Extraction to Information. The Process. Step 1: DNA Extraction Genomic DNA extraction from the organism ( bacteria ). Step 2: PCR Amplification Amplification of the DNA segment of interest ( 16S gene ). Step 3: DNA Sequencing
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AFLP and microsatellite analysis. Amplified Fragment Length Polymorphism. Pros: Large number of markers with relatively little lab effort No prior information about genome needed Genome wide overage Small amount of DNA needed Cons:
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Genetic and Molecular Epidemiology. Lecture III: Molecular and Genetic Measures Jan 19, 2009 Joe Wiemels HD 274 (Mission Bay) 514-0577 email@example.com. Lecture . Types of genetic markers: SNPs and microsatellites Assessing genetic markers
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Design and Verification Tools for Continuous Fluid Flow-based Microfluidic Devices. Jeffrey McDaniel, Auralila Baez, Brian Crites, Aditya Tammewar , Philip Brisk University of California, Riverside. Asia and South Pacific Design Automation Conference Yokohama, Japan, January 23, 2013.
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Hemoglobin Beta-subunit (HBB). Josh Bram and Wilton Smith. What is Hemoglobin?. Oxygen (and CO 2 ) transporter for all vertebrates Tetramer composed of four protein subunits (two alpha and two beta subunits) Four Iron-containing haeme centers carry one oxygen molecule each
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Utilizing Bioinformatics. Cloning and expression in E. coli. Useful Websites for Gene Cloning Using Bioinformatics. Portal sites Ecogene : http://ecogene.org/ Bioinformatics organization: http://www.bioinformatics.org/ Expasy : http://www.expasy.org/ NCBI: http://www.ncbi.nlm.nih.gov/
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Introduction to Sizing DNA on an Agarose Gel. HC 70AL Spring 2009 April 2, 2009 By Kristin Gill. How Do We Determine the Size of a PCR Product?. Fractionate a PCR product along with a DNA ladder by gel electrophoresis
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Cloning Vector Map. Vector Characteristics. Topoisomerase. Contains a T-overhang Linked topoisomerase. Topoisomerase. PCR Fragment Properties. A. PCR Product. A. Contains an A-overhang A-T base pairing helps anchor the insert to vector. Topoisomerase. PCR Product. A. A.
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Agarose Gel Electrophoresis . O. O. P. O. Base. O. O. CH 2. Sugar. Negative charges. O. O. O. P. Base. O. O. CH 2. Sugar. OH. Deoxyribonucleic Acid (DNA). Moves towards a positive charge . Agarose Matrix.
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plg-1 gene expressing propionicin by lactic starters in dairying. Arab Republic of Egypt. Alexandria University. Sameh E. Mohamed and Mahmoud K. Tahoun Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University.
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