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Department of Hydraulic and Environmental Engineering

Department of Hydraulic and Environmental Engineering.

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Department of Hydraulic and Environmental Engineering

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  1. Department of Hydraulic and Environmental Engineering Development of an ”at-line” sensor for monitoring bio-fouling potential in membrane filtration systems for drinking water treatment using ezyme activity measurements.Blanca Magdalena Gonzalez SilvaAdvisors: Cheng Sun, post doc; Stein W. Østerhus professor.

  2. Aim Development of an non-destructive early warning monitoring at-line of enzyme activity, as an indicator of biofouling potential in membrane filtration process.

  3. Introduction: Demand for on- line,non-destructive real-time information about biofilms in technical systems: Examples: Physical or physico-chemical on- line methods: a) Detect ↑or↓ of material accumulating on a surface (not ≠ biomass and other components of a deposit). • Most have not successful proof of operation at laboratory • None are specially designed for membrane biofouling monitoring b) Provide biological information and distinguish between biotic and abiotic material. c) Provide detailed chemical information.

  4. Introduction • For in-situ or at-line monitoring of membrane biofouling: Exo-enzymes Exo-hydrolases: hydrolyse organic compounds. Heterotrophic bacteria excellent producers of hydrolytic enzymes. • The activity of such exo-enzymes can therefore be used as a measure of total active biomass.

  5. How was done the enzymatic activity in previous experiments ? • Ultrasonic treatment  biofoulant removal • Enzyme activity assays

  6. 1.- Biofouling development 1.- Biofouling development permeate permeate 2.- Substrate injection (MUH) Raw water Raw water Substrate concentration= ≥ 0.02 mg MUH/mL Mixed time = 1 min Monitoring: Trans membrane pressure (TMP); Temperature Monitoring: Trans membrane pressure (TMP); Temperature Fluorimeter Flow=1 mL/min Strategy for carried out the development of an ”at- line” sensor: 3.- Enzyme measurement

  7. MUH: 4-methylumbelliferyl heptanoate MU: fluorescent 4-methylumbelliferone Fluorimeter Flow=1 mL/min 3.- Enzyme measurement Sofware : Clarity lite

  8. Day 10 Fluorescence Day 5 Day 1 Time (min) Expected results Enzymatic activity = Amount of 4methylbumbelliferone (MU) per minute per litre. Activity per cm2 membrane surface = μmol of MU realised per minute, per cm2.

  9. Enzyme activity Biomass/Biofouling Expected results • Understanding biofouling behaviour and responses: • to choose optimal operating conditions for biofouling mitigation and • control. It is assumed that cleaning can be more efficient when biofouling is in a early stage of colonisation

  10. Initial determinations and preparations Parameters: Volumen =440mL,Total membrane area (outside) = 0.015m2; Flux =20 L/m2*h; Flow valule= 5.0mL/min; Initial preparations • Familiarization with the fluorescence detector. • Determination of fluorescence signal with MU stock solution. Familiarization with the software : Clarity chromatography station Tests to find out how the software works

  11. Tasks / activities: Test and calibrate unit for enzyme measurement Perform experimental plan with small-scale test modules. Analysis and reporting of experimental results. Test at-line enzyme measurement with pilot scale membrane filtration systems.

  12. Thank you for your attention!!

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