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Quenching de fluorescencia

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Quenching de fluorescencia

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    1. Quenching de fluorescencia quenching = desactivacin= apagamiento = menor emisin IF (Q) < IF (-Q) Reacciones en el estado excitado (Quenching colisional) Entrecuzamiento intersistema a estado Triplete Rearreglos moleculares Transferencia de energa Transferencia de carga Formacin de complejo en el estado basal (Quenching esttico)

    9. Quenching dinmico o colisional Se requiere contacto entre Q y F, interaccin molecular Quenching dinmico o colisional: Q + F* Q debe difundir hacia F distancias moderadas (a diferencia de relajacin por solvente)

    26. Eficiencia FRET

    27. Formacin de agregados moleculares

    28. Usos de fluorescencia en bioqumica Localizacin subcelular Cambios en la concentracin Interacciones moleculares Cambios conformacionales Distancias intra/intermoleculares Difusin rotacional Caracterizacin estructural Actividad enzimtica ???

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