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Amino Acid Enzymes

Amino Acid Enzymes. Amino Acid Enzymes. Enzymes that attack Amino Acids 1. Cysteine desulfhydrase 2. Lysine decarboxylase 3. Phenylalanine deaminase. Amino Acid Structure. Amino Acid Enzymes. Cysteine desulfhydrase

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Amino Acid Enzymes

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  1. Amino Acid Enzymes

  2. Amino Acid Enzymes • Enzymes that attack Amino Acids 1. Cysteine desulfhydrase 2. Lysine decarboxylase 3. Phenylalanine deaminase

  3. Amino Acid Structure

  4. Amino Acid Enzymes Cysteine desulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvic acid

  5. Peptone Iron Agar Deeps • Peptone – protein rich in cysteine • Iron - Fe

  6. Cysteinedesulfhydrase Cysteine Hydrogen Sulfide + ammonia + Pyruvicacid H2S Fe (Iron) FeS (Ferrous Sulfide) Black

  7. Cysteine desulfhydrase

  8. Amino Acid Enzymes Lysine decarboxylase Lysine Cadaverine + Carbon dioxide

  9. Lysine Decarboxlyase Broth • Lysine • Fermentable sugar • ph indicator • Basic = purple • Acid = Yellow • Durham Tube • Mineral Oil on top after inoculation • (maintain an Anaerobic environment)

  10. Lysine decarboxylase broth

  11. Amino Acid Enzymes Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia

  12. Phenylalanine deaminase Phenylalanine Phenylpyruvic acid + ammonia FeCl2 (Ferric Chloride) HCl Green

  13. Phenylalanine Agar Slants

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