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Recovery of ZF by PCR

Recovery of ZF by PCR. FLAG+. FLAG-. RNA interference. May 9. siRNA. discovery pathway DICER and RISC software for siRNA design databases delivery of siRNA testing siRNA designing and testing siRNA against ZF. suppression of ZF by siZF. 10ng. 100ng. 500ng. pcZF. psiZF#14 µg. 0.

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Recovery of ZF by PCR

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  1. Recovery of ZF by PCR FLAG+ FLAG-

  2. RNA interference May 9

  3. siRNA • discovery • pathway DICER and RISC • software for siRNA design • databases • delivery of siRNA • testing siRNA • designing and testing siRNA against ZF

  4. suppression of ZF by siZF 10ng 100ng 500ng pcZF psiZF#14 µg 0 0.5 1 0 0.5 1 0 1 0.5 1 0.5 1 psiRad9 µg 1 2 3 4 5 6 7 8 9 10 11 14

  5. U6 prom GFP mU6pro BbsI XbaI PolIII term BspI TTTG…………TTTT …………AAAAGATC XbaI si hairpin

  6. siRNA for ZF ATGGAGGAGGAGGCGATCGCCAGCCTCCCGGGGGAAGAGACGGAGGATATGGACTTTCTGTCTGGGCTGGAACTGGCGGATCTCCTGGACCCCAGGCAACCGGACTGGCACCTGGACCCCGGGCTTAGCTCGCCGGGGCCTCTCTCCTCGTCTGGCGGAGGCTCGGATAGCGGCGGCCTGTGGAGAGGGGACGATGACGATGAGGCCGCGGCTGCTGAAATGCAGCGCTTCTCTGACCTGCTGCAAAGGCTGTTAAACGGTATCGGAGGCTGCAGCAGCAGCAGTGACAGTGGCAGCGCCGAAAAGAGGCGGAGAAAGTCCCCAGGAGGAGGCGGCGGTGGCGGCAGCGGTAACGACAACAACCAGGCGGCGACAAAGAGTCCCCGGAAGGCGGCGGCGGCCGCTGCCCGCCTTAATCGACTGAAGAAGAAGGAGTACGTGATGGGGCTGGAGAGTCGAGTCCGGGGTCTGGCAGCCGAGAACCAGGAGCTGCGGGCCGAGAATCGGGAGCTGGGCAAACGCGTACAGGCACTGCAGGAGGAGAGTCGCTACCTACGGGCAGTCTTAGCCAACGAGACTGGACTGGCTCGCTTGCTGAGCCGGCTGAGCGGCGTGGGACTGCGGCTGACCACCTCGCTCTTCAGAGACTCGCCCGCCGGTGACCACGACTACGCTCTGCCAGTGGGAAAGCAGAAGCAGGACCTGCTGGAAGAGGACGACTCGGCGGGAGGAGTCTGTCTCCATGTGGACAAGGATAAGGTGTCGGTGGAGTTCTGCTCGGCGTGCGCCCGGAAGGCGTCGTCTTCTCTTAAAATGTAG #14 #15

  7. oligos

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