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Write in planner!

17. 18. 19. Write in planner!. You need: A pencil DNA note sheets and reading sheets Today: DNA replication. HW: Due Fri. 01/18/08 Write a results section and conclusion section for the Neurospora experiment Must be typed!. What makes up the DNA backbone?.

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Write in planner!

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  1. 17 18 19 Write in planner! • You need: • A pencil • DNA note sheets and • reading sheets • Today: • DNA replication HW: Due Fri. 01/18/08 Write a results section and conclusion section for the Neurospora experiment Must be typed!

  2. What makes up the DNA backbone?

  3. Which parts of the nucleotides are connected to make one strand (half) of DNA? • Which parts of the nucleotides connect the two strands (halves) of DNA? • What type of bond connects the two strands together?

  4. What types of molecules are necessary for DNA replication? • Which enzyme unwinds DNA? • Where does it unwind the DNA? • Which enzyme connects new nucleotides to the DNA during replication?

  5. Complementary base pairing • If one strand of DNA has the following sequence, CTAATGT GATTACA What is the base sequence (order of nucleotides) for the complimentary strand?

  6. Enzymes in DNA replication • Enzymes are proteins that act as machines in the cell

  7. Enzymes in DNA replication • Enzymes are proteins that act as machines in the cell • 2 enzymes do the work of DNA replication: • Helicase is responsible for separating the 2 strands of DNA • DNA Polymerase is responsible for adding on new Nucleotides. We will replicate (copy) our DNA molecule in class to produce 2 new identical DNA molecules

  8. Helicase • Helicase unwinds and separates the 2 strands of DNA so that each strand may be copied. • Helicase breaks the hydrogen bonds between the bases of each strand. Hydrogen Bonds

  9. DNA Polymerase • DNA Polymerase adds on the complimentary bases to the new strands of DNA • DNA Polymerase makes bonds between the phosphate and the deoxyribose sugar of the nucleotides. T A Phosphate-Sugar bond

  10. DNA replication C T A G G T T C C

  11. Replication in Action • Replication occurs simultaneously at multiple places along a DNA strand. • 1 chromosome is about 80 million base pairs long. • Replication is as fast as 50 base pairs a second! Nobel e-Museum: DNA – leading strand replication Nobel e-Museum: DNA – lagging strand replication

  12. Complicated picture of replication…

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