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This report details the development of constructs designed for studying quorum sensing and cell-cell signaling in microbial systems. It focuses on sender and receiver constructs in single-cell (‘JT’) and two-cell configurations. The signaling mechanisms utilize the R.OHHL reporter system and target beads to facilitate communication between cells. These constructs are essential for investigating the dynamics of microbial interactions and understanding the underlying processes of quorum sensing, enabling researchers to explore synthetic biology applications in cell signaling.
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luxI/luxR Quorum Sensing Cell-Cell Signalling Reporter Target (bead) Receiver + Sender R OHHL
Cell-Cell Signaling:Constructs Sender Receiver Single Cell Construct – “JT” Two Cell Construct Receiver Sender
Cell-Cell Signaling:Single Cell Construct (“JT”) Target (bead)
Cell-Cell Signaling: Two Cell Construct Sender Receiver Target (bead)