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Translation

Translation. Cien años de soledad. One hundred years of solitude. AUGGGGUUUGGC. MGUG. polypeptide. mRNA. What’s needed?. mRNA. tRNAs. ribosome. open reading frame. mRNA. many aa codons. cap. UAG. AUG. (A) n. size usually 500 - 5000 nt. tRNA. acceptor arm. about 80 nt.

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Translation

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  1. Translation Cien años de soledad One hundred years of solitude AUGGGGUUUGGC MGUG polypeptide mRNA

  2. What’s needed? mRNA tRNAs ribosome

  3. open reading frame mRNA many aa codons cap UAG AUG (A)n size usually 500 - 5000 nt

  4. tRNA acceptor arm about 80 nt contains unusual bases pseudouracil () D loop dihydrouracil (D)  loop (T) methyluracil anticodon loop Kenyon College

  5. Essential Biochemistry Wiley Kenyon College Thinkquest

  6. amino acid aa tRNA synthetase charging of tRNAs aminoacyl tRNA carboxyl group of aa connected to 3’ OH of tRNA

  7. arginyl tRNA Arg + tRNA Arg tRNA synthetase specific for one aa and its correct tRNAs specificity of aa tRNA synthetases many amino acids have more than one tRNA

  8. each mRNA may have several ribosomes on it (polyribosome = polysome) rRNAs  half of all RNA in the cell # of ribosomes very large ( 106 in a large cell)

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