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Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies

GEIL-GTLLF 2008. Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies. Part two : Granulocytic maturation. II.1. SSC. SSC. SSC. SSC. SSC. SSC. FLy. FLy. FLy. FLx. FLx. CD45. FLn. CD45. FLx. CD45. CD45. CD45. Backgating. Display.

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Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies

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  1. GEIL-GTLLF 2008 Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies Part two : Granulocytic maturation II.1

  2. SSC SSC SSC SSC SSC SSC FLy FLy FLy FLx FLx CD45 FLn CD45 FLx CD45 CD45 CD45 Backgating Display Strategy Granulober II.2

  3. SSC SSC SSC SSC SSC FL3 FL3 FL2 CD45 CD45 FL1 CD45 FL2 CD45 FL1 CD45 Crossgating FLn+ SSC FLn Display Strategy Granulober FL2+ FL1+ FL3+ II.3

  4. Informative combinations Tube 1 : CD11b/CD14/CD16/CD45 Tube 5 : CD38/DR/CD34/CD45 CD11b/CD14 p. II-5 CD38/HLADR p. II.20 CD11b/CD16 p. II-6 CD38/CD34 p. II.21 Crossgating p. II-7 Crossgating p. II.22 Tube 2 : CD11b/CD117/CD34/CD45 Tube 7 : DR/CD117/CD34/CD45 CD11b/CD117 p. II-8 HLADR/CD117 p. II.23 CD11b/CD34 p. II-9 HLADR/CD34 p. II.24 CD117/CD34 p. II-10 CD117/CD34 p. II.25 Crossgating p. II-11 Crossgating p. II.26 Tube 3 : CD36/CD71/CD11c/CD45 Tube 9 : CD64/CD24/CD10/CD45 CD36/CD71 p. II-12 CD64/CD24 p. II.27 CD36/CD11c p. II-13 CD64/CD10 p. II.28 CD71/CD11c p. II-14 CD24/CD10 p.II29 Crossgating p. II-15 Crossgating p. II.30 Tube 4 : CD34/CD13/CD33/CD45 CD34/CD13 p. II-16 CD34/CD33 p. II.17 CD13/CD33 p. II.18 Crossgating p. II.19 II.4

  5. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD14PE CD14PE CD14PE CD11bFITC CD11bFITC CD11bFITC CD11b/CD14 Granulober Gating CD14 Gating CD11b II.5

  6. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD16PE CD16PE CD16PE CD11bFITC CD11bFITC CD11bFITC CD11b/CD16 Granulober Gating CD16 Gating CD11b II.6

  7. SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL2 FL3 FL3 CD16PC5 CD16PC5 CD14PE FL1 FL1 FL2 CD11bFITC CD11bFITC CD14PE Crossgating CD11b/CD14/CD16 Gating CD16 Gating CD11b Gating CD14 II.7

  8. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD117PE CD117PE CD117PE CD11bFITC CD11bFITC CD11bFITC CD11b/CD117 Granulober Gating CD117 Gating CD11b II.8

  9. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD34PC7 CD34PC7 CD34PC7 CD11bFITC CD11bFITC CD11bFITC CD11b/CD34 Gating CD34 Gating CD11b Granulober II.9

  10. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD34PC7 CD34PC7 CD34PC7 CD117PE CD117PE CD117PE CD117/CD34 Granulober Gating CD117 Gating CD34 II.10

  11. SSC-Height SSC-Height SSC-Height CD45APC CD45APC CD45APC SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL2 FL3 FL3 CD34PC7 CD34PC7 CD117PE FL1 FL1 FL2 CD11bFITC CD11bFITC CD117PE Crossgating CD11b/CD117/CD34 Gating CD34 Gating CD11b Gating CD117 II.11

  12. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD71PE CD71PE CD71PE CD36FITC CD36FITC CD36FITC CD36/CD71 Granulober Gating CD36 Gating CD71 II.12

  13. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD11cPC5 CD11cPC5 CD11cPC5 CD36FITC CD36FITC CD36FITC CD36/CD11c Granulober Gating CD36 Gating CD11c II.13

  14. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD11cPC5 CD11cPC5 CD11cPC5 CD71PE CD71PE CD71PE CD71/CD11c Granulober Gating CD71 Gating CD11c II.14

  15. SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ CD11cPC5 CD11cPC5 FL2 FL3 FL3 CD71PE FL1 FL1 FL2 CD36FITC CD36FITC CD71PE Crossgating CD36/CD71/CD11c Gating CD11c Gating CD36 Gating CD71 II.15

  16. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD13PE CD13PE CD13PE CD34FITC CD34FITC CD34FITC CD34/CD13 Granulober Gating CD34 Gating CD13 II.16

  17. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD33PC5 CD33PC5 CD33PC5 CD34FITC CD34FITC CD34FITC CD34/CD33 Granulober Gating CD34 Gating CD33 II.17

  18. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD33PC5 CD33PC5 CD33PC5 CD13PE CD13PE CD13PE CD13/CD33 Granulober Gating CD13 Gating CD33 II.18

  19. SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL2 FL3 FL3 CD33PC5 CD33PC5 CD13PE FL1 FL1 FL2 CD34FITC CD34FITC CD13PE Crossgating CD34/CD13/CD33 Gating CD33 Gating CD34 Gating CD13 II.19

  20. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD HLADRPE HLADRPE HLADRPE CD38FITC CD38FITC CD38FITC CD38/HLADR Granulober Gating CD38 Gating HLADR II.20

  21. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD34PC5 CD38FITC CD38FITC CD38FITC CD38/CD34 Granulober Gating CD38 Gating CD34 II.21

  22. SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ HLADRPE FL2 FL3 FL3 CD34PC5 CD34PC5 FL1 FL1 FL2 CD38FITC CD38FITC HLADRPE Crossgating CD38/HLADR/CD34 Gating CD34 Gating CD38 Gating HLADR II.22

  23. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD117PE CD117PE CD117PE HLADRFITC HLADRFITC HLADRFITC HLADR/CD117 Granulober Gating HLADR Gating CD117 II.23

  24. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD34PC5 HLADRFITC HLADRFITC HLADRFITC HLADR/CD34 Granulober Gating HLADR Gating CD34 II.24

  25. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD34PC5 CD117PE CD117PE CD117PE CD117/CD34 Gating 117 Granulober Gating CD34 II.25

  26. SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL2 FL3 FL3 FL1 FL1 FL2 Crossgating HLADR/CD117/CD34 Gating CD34 Gating HLADR Gating CD117 II.26

  27. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45PC7 CD45PC7 CD45PC7 CD24PE CD24PE CD24PE CD64FITC CD64FITC CD64FITC CD64/CD24 Granulober Gating CD64 Gating CD24 II.27

  28. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45PC7 CD45PC7 CD45PC7 CD10PC5 CD10PC5 CD10PC5 CD64FITC CD64FITC CD64FITC CD64/CD10 Gating CD10 Granulober Gating CD64 II.28

  29. SSC SSC SSC CD45 CD45 CD45 SSC-Height SSC-Height SSC-Height FLy FLy FLy FLx FLx FLx CD45PC7 CD45PC7 CD45PC7 CD10PC5 CD10PC5 CD10PC5 CD24PE CD24PE CD24PE CD24/CD10 Gating CD10 Granulober Gating CD24 II.29

  30. SSC-Height SSC-Height SSC-Height CD45PC7 CD45PC7 CD45PC7 SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL2 FL3 FL3 CD10PC5 CD10PC5 CD24PE FL1 FL1 FL2 CD64FITC CD64FITC CD24PE Crossgating CD64/CD24/CD10 Gating CD10 Gating CD64 Gating CD24 II.30

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