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Enhancing Energy Transfer Quantification in Bacterial Membranes Using Innovative Chamber Design

This project focuses on precise quantification of energy transfer at the single-cell level, specifically examining the spatial and temporal dynamics of cytochromes in bacterial membranes. Guided by Jan Liphardt from UCB Biophysics, we aim to perfect the sealing of all-glass chambers using SU-8 for channel formation and UV epoxy for bonding. The integration of thin electrode wafers allows for higher magnification and the addition of reference electrodes post-assembly. Current efforts involve short-term tests with Ag/AgCl electrodes and biofilm impedance analyses.

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Enhancing Energy Transfer Quantification in Bacterial Membranes Using Innovative Chamber Design

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Presentation Transcript

  1. Long term goal • “Single cell” energy transfer quantification • Spatial and temporal location of energy transfer (cytochromes) in bacterial membrane • Jan Liphardt (UCB Biophysics prof)

  2. Goals: Perfecting chamber Sealing (all glass/no PDMS)  Use SU8 for channels and UV epoxy for SU8/glass bonding Thin electrode wafer for higher magnification Addition of reference electrode Will be implementing capillary/agar/silver electrode added after assembly (no microfabrication) Short term Silver wire Agar junction Tip into chip Ag/AgCl reference electrode

  3. All have passed individual tests Proposed architecture Short term results glass SU8 ITO ITO glass

  4. Right now • Mask for MEMS • My chamber for few cell signal • Adrienne’s biofilm impedance

  5. Other news • Nikkei Microdevices • Put us on p. 3 • Didn’t include any names! • IAB • ARPA E – any news?

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