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Differential MMP Expression in Cardiac Tissue Before and After CPB: A Comparative Western Blot Study

Protein expression of MMP-2/9 in cardiac tissue pre- and post-CPB analyzed using Western blotting. Results suggest a differential regulation of MMPs with Actin as reference. A representative blot from control and RIPC patients displayed. SEM shown.

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Differential MMP Expression in Cardiac Tissue Before and After CPB: A Comparative Western Blot Study

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  1. After CPB Before CPB N=17 N=18 N=17 N=18 B A N=17 N=18 N=17 N=18 D C MMP-9 MMP-9 MMP-2 MMP-2 Actin Actin RIPC RIPC Control Control F E Supplemental Figure 2 Proteinexpression of MMP-2/9. Westernblotting experiments were performed using cardiac tissue derived before CPB (A, C) and after CPB (B, D). Antibodies against MMP-2 (A, B) and MMP-9 (C, D) were employed and the intensity of the respective signal was related to the intensity of actin. One representative westernblot containing samples from 6 control and 6 RIPC patients is shown in E (before CPB) and F (after CPB). Bars denote SEM.

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