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Western blot analysis depicts the accumulation of mutant protein Δ(194-201) during viral infection in N. benthamiana plants compared to wild-type CP. Despite an 8-amino acid deletion, the stability of the mutant protein remained unchanged. Total protein loading was confirmed via Ponceau staining.
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Δ(194-201) Mock CP 26 kDa - CP Ab 17.3kDa - FigS.1 Western blot showing the accumulation of the mutant protein Δ(194-201) during viral infection in N. Benthamiana plants compared with wt CP. Despite the deletion of 8 amino acids, the stability of the mutant Δ(194-201) was not altered. Equal total protein loading was cheked by Ponceau staining.