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Optical microscopy

MENA3100, 26/1-10. Optical microscopy. Magnification Resolution Contrast. We want to see more details. Simple microscope. The simple microscope. Antony van Leeuwenhoek. (1632−1723). The compound microscope. ca. 1595 Hans Janssen. Robert Hooke (1635−1703). Micrographia , 1664.

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Optical microscopy

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  1. MENA3100, 26/1-10 Optical microscopy Magnification Resolution Contrast We want to see more details Simple microscope

  2. The simple microscope Antony van Leeuwenhoek (1632−1723)

  3. The compound microscope ca. 1595 Hans Janssen Robert Hooke (1635−1703) Micrographia, 1664

  4. Don’t get too hang up in the ray tracing

  5. Stereo microscope

  6. Compound microscope for transmitted light

  7. Inverted compound microscope for reflected light

  8. www.microscopy-analysis.com/files/ jwiley_microscopy/2006_Sept_Hammond.pdf

  9. Illuminating system Specimen Condenser lens Aperture diaphragm Field diaphragm Filament

  10. Illuminating system Specimen Condenser lens Aperture diaphragm Köhler illumination Uniform specimen illumination Field diaphragm Controll illuminated area Field diaphragm Aperture diaphragm Controll angle of the cone of light Filament

  11. Imaging system Eyepiece Okular Tube lens Objective Specimen

  12. Primary image

  13. Refraction Kromatisk abberation

  14. Spherical abberation

  15. The objective Corrections

  16. Numerical aperture (NA) NA = nsina Resolution R = 0,61l/NA Rayleigh criterion (Raleigh in Brandon and Kaplan)

  17. The limiting factor: Diffraction NA = nsina Oil immersion

  18. More is needed than just the objective Condenser system Eyepice Ocular (okular)

  19. Optical anisotropy Calcite

  20. Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices Lower symmetry: Optically anisotropic, three refractive indices

  21. Optically active crystals in the microscope

  22. Optically active crystals in the microscope

  23. Michel-Levy chart

  24. Remember that geologists often call their book on microscopy on thin sections for optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi). Some web sites: http://www.olympusmicro.com/primer/ http://www.microscopyu.com/ http://www.micro.magnet.fsu.edu/ http://www.micro.magnet.fsu.edu/primer/pdfs/basicsandbeyond.pdf maintenance: http://www.zeiss.de/C1256D18002CC306/0/1205E8CBD68054EAC125701500404705/$file/46-0009_e.pdf

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