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Important Announcement for Exam 3 on March 28 - Key Material and Guidelines

Exam 3 is scheduled for Wednesday, March 28. Ensure you review Lectures 19-25 and Key Terms #7-9 (cancer lecture materials will be posted later today). Homework 6-7 will be included in the exam (note: Homework 5 will not). The exam format will be similar to previous assessments in BIO 304. Key topics include the lac operon, types of enzymes (inducible, constitutive, repressible), and gene regulation mechanisms. Be prepared to analyze genetic scenarios for E. Coli in relation to lactose presence.

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Important Announcement for Exam 3 on March 28 - Key Material and Guidelines

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  1. Announcements • Exam 3 is Wednesday, 3/28 • Material for Exam 3: • Lectures 19-25 • Key terms #7-9 (key terms 9 are from cancer lecture; will be posted later today) • Homeworks 6-7 (NOT homework 5) • Same format as previous exam

  2. BIO 304 Lecture 26 Lac repressor bound to DNA

  3. Regulation of gene expression

  4. Some terminology Inducible enzyme: Constitutive enzyme: Repressible enzyme: Positive gene regulation: Negative gene regulation: Cis-acting site: Trans-acting factor:

  5. The lac operon

  6. The lac operon

  7. The lac operon

  8. In the lac operon model, which of these components is a cis-acting element? 1. The lac repressor ✓ 2. The lac operator 3. The beta-galactosidase enzyme 4. Lactose

  9. Genetic proof of the operon model

  10. For the following E. Coli genotypes, determine what the phenotypes would be in the presence of lactose: inducible, permanently repressed, or constitutively active? 1. I+ O+ Z+ 2. I- O+ Z+ 1. inducible; inducible 2. repressed; constitutive ✓ 3. inducible; constitutive 4. repressed; inducible 5. constitutive; repressed

  11. Positive control of the lac operon

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