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Extensive affinity maturation is critical to neutralization effectiveness of Abs

This study examines the affinity maturation and neutralization effectiveness of antibodies in long-term non-progressors (LTNPs) with HIV-1. Results show higher levels of elite neutralizers and broader neutralization spectra in LTNPs compared to progressors, suggesting preserved B cell function. Some LTNPs have multiple antibody specificities contributing to neutralization breadth, with a higher prevalence of glycan-dependent NAbs. The research highlights the potential of LTNPs to produce bNAbs with increased affinity, providing insights into HIV-1 immunity.

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Extensive affinity maturation is critical to neutralization effectiveness of Abs

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  1. Characterization of broadly neutralizing antibodies to HIV-1 present in a cohort of long term non-progressors (LTNPs) N González, K McKee, E Yuste, J R. Mascola and J Alcamí • Extensive affinity maturation is critical to neutralization effectiveness of Abs • ART patients induce a humoral immune response of broad spectrum • Preserved B cell function in LTNPs could result in the production of bNAbs at higher frequency and increased affinity in comparison with progressors. Recombinant viruses + 1 hour incubation Serum (dilutions 1/200 1/2000) U87.CD4.CCR5/CXCR4 LUCIFFERASE ACTIVITY 48 hours Background. Material and methods

  2. Neutralizing activity • The percentage of elite neutralizers was higher in the LTNPs than in the normal progressors. Neutralization breadth of sera from LTNPs Results

  3. Summary of serum neutralization specificities POSTER #TUPDA0104 • bNAbs in LTNPs can be mapped to specific known neutralization epitopes. • In some subjects neutralization breadth was mediated by more than one antibody specificity. • Glycan-dependent HIV-1 NAbs were more abundant in LTNPs (9/12) than in normal progressors (3/7).

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