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Baculovirus-Mediated Transgene Expression in Nude Mice Brain

Baculoviral vectors with HSV thymidine kinase gene injected into nude mice striatum, brain collected and stained 2 days post-injection, images captured under a fluorescence microscope. (a) Low power image showing transduced region. (b) High power image displaying entire region in indicated box. (c) Highly magnified region indicating baculovirus spread of at least 250 µm.

courtney-erickson
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Baculovirus-Mediated Transgene Expression in Nude Mice Brain

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  1. Supplementary Figure 1. a b c b Baculovirus-mediated transgene expression in the brain of nude mice. Baculoviral vectors containing the HSV thymidine kinase gene (107 plaque-forming units) were injected into the striatum of Balb c/Nude mice. The brain was collected 2 days post-injection and stained with antibody against the HSV thymidine kinase. Images were taken under a fluorescence microscope. (a) A low power image to show the position of the transduced region. (b) A high power image to show the whole region in the box indicated in (a). (c) A highly magnified region of the transduced tissue indicates a spread of baculovirus of at least 250 µm.

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