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Methylation Levels of LINE1 and Alu in ESCC Patients

Investigating methylation levels of LINE1 and Alu elements in esophagus cancer (ESCC) patients compared to normal individuals using supplementary figures and statistical analysis.

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Methylation Levels of LINE1 and Alu in ESCC Patients

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  1. 90 85 80 75 70 65 40 44 48 52 56 90 80 70 60 50 38 40 42 44 46 48 50 52 90 80 70 60 50 40 30 25 30 35 40 45 50 55 Normal r = 0.043 p = 0.678 Methylation Level of LINE1 (%) Background r = 0.063 p = 0.559 Methylation Level of LINE1 (%) ESCC r = 0.502 p < 0.001 Methylation Level of LINE1 (%) Methylation Level of Alu (%) Supplementary Figure 1

  2. a b HOXA9 NEFH p < 0.001 p < 0.001 p < 0.001 p < 0.001 100 60 50 p < 0.001 80 40 60 Methylation Level (%) 30 40 20 p = 0.276 20 10 0 0 ESCC Normal Background ESCC Normal Background (n = 89) (n = 93) (n = 95) (n = 89) (n = 93) (n = 95) c d UCHL1 MT1M p < 0.001 p < 0.001 p < 0.001 p < 0.001 100 80 80 60 60 p = 0.018 Methylation Level (%) 40 40 p = 0.118 20 20 0 0 ESCC ESCC Normal Background Normal Background (n = 89) (n = 93) (n = 89) (n = 93) (n = 95) (n = 95) Supplementary Figure 2

  3. a b c Supplementary Figure 3

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