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Supplementary Figure 1. Aggregation state of A1-42 was measured by thioflavin T fluometric assay in a cell-free system. ThioflavinT spectroscopic assay was carried out from 0 to 5 days at 37 C. After A1-42 or non-containing samples (0 – 5 days) were added to 50 M Thioflavin-T solution, fluorescence was measured at 450 nm excitation and 482 nm emission using a fluorescence spectrometer. Each sample was determined in triplicates.