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Target Gene

Supplemental figure 1. a. FvTox1 gene structure. Promoter region. Terminator region. Target Gene. Terminator region. Promoter region. pRF-HU2. hph. RB. LB. Supplemental figure 1. b. FvTox1 probe. T-I-F. X. X. E. T-I-R. T-O1. T-O2. T-O3. T-O4. FvTox1. Terminator.

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Target Gene

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  1. Supplemental figure 1. a FvTox1 gene structure Promoter region Terminator region Target Gene Terminator region Promoter region pRF-HU2 hph RB LB

  2. Supplemental figure 1. b FvTox1 probe T-I-F X X E T-I-R T-O1 T-O2 T-O3 T-O4 FvTox1 Terminator Promoter 7,643bp Integration of the hph cassette In genome X X X E X E hph hph probe Promoter Probe Terminator Probe Hyg588U Hyg588L T-U T-D RF-2 RF-1 6,600 bp XbaI DNA fragment to be detected by the Promoter probe DNA fragment to be detected by the Terminator probe 4,500 bp XbaI & EcoRI 4,500 bp 1,227 bp 3,300 bp

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