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Palindrome, Restriction Enzyme, Sticky Ends. CIVIC, Madam. AATTC. G. GAATTC. AATTC. G. GAATTC. G. AATTC. AATTC. G. G et A n A pple T o T he C lass. Arber, Nathans, Smith (1978). Sticky Ends (Cohesive Ends). EcoRI. Juang RH (2004) BCbasics. Restriction Mapping of DNA. 8 kb
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Palindrome, Restriction Enzyme, Sticky Ends CIVIC, Madam AATTC G GAATTC AATTC G GAATTC G AATTC AATTC G Get An Apple To The Class Arber, Nathans, Smith (1978) Sticky Ends (Cohesive Ends) EcoRI Juang RH (2004) BCbasics
Restriction Mapping of DNA 8 kb 2 kb A 7 kb 3 kb B 5 kb 3 kb 2 kb A + B Restriction enzymes A B 10 kb CK A B A+B M Juang RH (2004) BCbasics
The Specific Cutting and Ligation of DNA GAATTC GAATTC CTTAAG CTTAAG G G G G G AATTC AATTC AATTC AATTC CTTAA CTTAA CTTAA CTTAA CTTAA G G G G AATTC G EcoRI EcoRI sticky end EcoRI sticky end DNA Ligase Juang RH (2004) BCbasics