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Comparative Expression of BnSTM in Brassica napus MDEs under BSO and TIBA Treatments

This study investigates the expression levels of BnSTM in developing Brassica napus MDEs cultured in the presence or absence of DL-buthionine sulfoximine (BSO) and 2,3,5-triodobenzoic acid (TIBA). Samples were analyzed at days 7, 14, and 21 following compound addition at day 5. Results include RNA in situ hybridization illustrating differences in expression due to treatment conditions. Values represent means with standard error derived from three biological replicates. The impact of these treatments on gene expression provides insights into plant developmental processes.

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Comparative Expression of BnSTM in Brassica napus MDEs under BSO and TIBA Treatments

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  1. 15 BnSTM a 10 BSO Relative expression control TIBA 5 Control BSO TIBA 5 7 14 21 28 0 Days in culture b Day 7 Day 14 Day 21 Day 7 Day 14 Day 21 Day 7 Day 14 Day 21 Supplemental Fig. S2. (a) Expression level of BnSTM in developing Brassica napus MDEs cultured in the absence (control) or presence of DL-buthioninesulfoximine (BSO) or 2,3,5-triodobenzoic acid (TIBA). Compounds were added at 5 days in culture. Values + SE are means of three biological replicates. (b) RNA in situ hybridization of BnSTM in developing MDEs treated with BSO or TIBA. Scale bars = 6 mm

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