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MANIPULATION OF WHOLE BLOOD USING TRAVELING WAVE DIELECTROPHORESIS

MANIPULATION OF WHOLE BLOOD USING TRAVELING WAVE DIELECTROPHORESIS. Y.J. Lo, A.M. Wo, and U. Lei Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan (MEMS2005 P.686~689) Reporter: Chung-Kuang Chin( 秦重光 ) Date:10/24/2007. Outline. Motivation Introduction

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MANIPULATION OF WHOLE BLOOD USING TRAVELING WAVE DIELECTROPHORESIS

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  1. MANIPULATION OF WHOLE BLOOD USING TRAVELING WAVE DIELECTROPHORESIS Y.J. Lo, A.M. Wo, and U. Lei Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan (MEMS2005 P.686~689) Reporter: Chung-Kuang Chin(秦重光) Date:10/24/2007

  2. Outline • Motivation • Introduction • Working Theory of DEP/twDEP • Fabrication • Experiment Results

  3. Motivation • Beautiful Life, Money－＞Health Care －＞Bio-Medical Science + MEMS －＞Bio-MEMS ( “Lab on a Chip”，”Micro-total analysis system” ) －＞cell manipulation(μm) －＞DEP force (working on whole blood is rare) －＞This paper !!!! (MANIPULATION OF WHOLE BLOOD USING TRAVELING WAVE DIELECTROPHORESIS ) －＞But after Reading，…@#&%*! Ref: http://www.imm-mainz.de/upload/bilder/d137027ad17056118590d74d1c2b8b30.orig.jpg

  4. Introduction • DEP－conventional DEP (cDEP) 、rotation DEP (rotDEP) 、traveling-wave DEP (twDEP) 10MHz

  5. Theory－DEP/twDEP • DEP force －factor：non-uniform electric field、electrical properties of particles & medium Ref: http://www.ibmm.informatics.bangor.ac.uk

  6. Theory Formula DEP force ( ，Clausius–Mossotti factor ε：permittivity， ， σ：conductivity ， ω：angular frequency ， r：particle radius ：rms value of the electric field strength， ， (i=x,y,z)：magnitude ，phase ) Real part－＞(Conventional) DEP ，Imaginary part－＞twDEP

  7. Clausius-Mossotti factor ( induced dipole moment factor)

  8. Clausius-Mossotti factor Amplitude & Phase plot of

  9. Particle Manipulation using DEP & twDEP Figure When a particle is suspended in an alternating electric field which contains either a magnitude or phase gradient, a force is induced on the particle which acts either in the direction of the gradient or opposes it, according to whether or not the particle is more or less Polari able than the medium in which it is suspended. Here, a particle experiences force due to (a) a non-uniformelectric field (magnitude gradient); (b) a traveling electric field (phase gradient

  10. Fabrication • Electrode (Cr) • Micro-channel (PDMS) • Bonding (O2 plasma) Ref: http://www.ndl.org.tw/old/ndlcomm/P12_1/3.pdf

  11. Experiment Results (1) • motion ofwhole blood cells being pumped by twDEP with phasing between electrodes

  12. Experiment Results (2) • Effect of electrode gap and voltage on average cell velocity Fdrag α Velocity FDEP α (Voltage)*2

  13. Experiment Results (3) • Bi-directional manipulation Fig. Computational result of twDEP force vectors

  14. THE END Thanks for Your Attention!

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