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Figure 1 D A B C * ** 2.5 4 6 5 Control Control Control Control *** *** * GW1929 GW1929 GW1929 GW1929 *** 5 4 2 RSG * RSG RSG 3 4 * 3 1.5 * Visfatin / cyclophilin mRNA * Visfatin / cyclophilin mRNA Visfatin / cyclophilin mRNA Visfatin / cyclophilin mRNA * ** * 3 2 *** 2 1 2 1 Control Control Control 1 0.5 RSG RSG RSG 1 3H 6H 9H 0 12H 24H AcLDL ATM * * * E F H G 3 7 *** *** *** Control * 4 3 6 GW1929 5 *** *** 3 2 *** Visfatin / cyclophilin mRNA CD36 / cyclophilin mRNA 4 FABP4 / cyclophilin mRNA 2 Visfatin / cyclophilin mRNA 2 3 § 1 1 2 1 1 T00709 AdGFP AdGFP AdGFP Control AdPPARg AdPPARg AdPPARg

A B Figure 2 1.6 3 Control Control GW1929 GW1929 1.4 RSG RSG * 1.2 * 2 1 mVisfatin / cyclophilin mRNA 0.8 mCD36 / cyclophilin mRNA 0.6 1 0.4 0.2 C D 3 1.4 Control Control *** GW1929 GW1929 1.2 2.5 1 2 0.8 CD36 / cyclophilin mRNA Visfatin / cyclophilin mRNA 1.5 0.6 1 0.4 0.5 0.2

A B (DR1-Visfatin PPRE)6 RXRa +PPARg 14 RXRa +PPARg Ab anti-PPARg Control ** Lysate Cold DR1 Visfatin- PPRE wt Cold DR1-Visfatin-PPRE mut PPARg Lysate RXRa 12 GW1929 gal x 1000 gal x 1000 10 8 §§ - - b b 6 RLU / 4 2 pSG5 pSG5-PPARg (DR1-consensus PPRE)6 70 Control ** 60 GW1929 50 §§§ gal x 1000 gal x 1000 40 - - b b 30 RLU / RLU / 20 * 10 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 pSG5 pSG5-PPARg DR1-consensus PPRE DR1-Visfatin-PPRE wt Figure 3

A B ** 2.5 7 Control Control ** *** GW1929 GW1929 6 ** 2 ** ns ** 5 *** * 1.5 §§ § Visfatin / cyclophilin mRNA 4 Visfatin / cyclophilin mRNA §§ 3 * 1 * 2 0.5 1 M2 RM IL-1b LPS TNFa RM Figure 4

GW1929 Control Visfatin A B b-actin 2.5 1.6 *** * 1.4 2 1.2 1.0 1.5 Visfatin secretion (ng/mL) Visfatin / b-actin 0.8 1 0.6 0.4 0.5 0.2 Control GW1929 Control GW1929 Figure 5

Figure 6 § * * B C A § * 140 * 140 140 Control Control Control * * * * GW1929 GW1929 GW1929 130 120 120 RSG * * 120 100 100 * 110 80 80 * * Intracellular NAD+ concentration (%) Intracellular NAD+ concentration (%) Intracellular NAD+ concentration (%) §§ § 100 60 60 90 40 40 80 20 20 scrambled siRNA visfatin AdPPARg vehicle AdGFP FK866

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