Figure 1

1. 150 100 50 0 1000 800 600 400 150 200 0 100 50 0 Figure 1 A FABP4 mRNA levels relative to WT SC controls * * WT shFABP4 WT shFABP4 HFD SC B FABP5 mRNA levels relative to WT SC controls WT shFABP4 WT shFABP4 HFD SC C FABP4 protein levels relative to WT SC controls * * WT shFABP4 WT shFABP4 HFD SC

2. * * * * * 30 HFD 25 * * * * * * * 20 * * * * 15 10 60 12 13 14 15 16 17 18 19 20 40 20 WT HFD WT HFD 0 shFABP4 HFD shFABP4 HFD 7 8 9 10 11 12 13 14 15 16 17 18 19 20 WT SC WT SC shFABP4 SC shFABP4 SC Figure 2 A Body weight (g) Age (weeks) B Food intake per week (g) * * * * * * * * Age (weeks)

3. 80 60 40 20 0 Figure 2 C * Percentage of fat weight/body weight WT shFABP4 WT shFABP4 HFD SC

4. 600 500 HFD WT HFD shFABP4 400 SC WT SC shFABP4 300 200 100 300 250 0 0 20 40 60 90 120 200 HFD WT HFD shFABP4 150 SC WT SC shFABP4 100 50 0 0 15 30 60 90 120 Figure 3 A Blood glucose (mg/dL) Minutes B Blood glucose (mg/dL) Minutes

5. 2.5 2.0 1.5 1.0 0.5 0.0 0.15 0.10 150 0.05 100 0 50 0 Figure 4 A LCFA desaturation index (C18:1/C18:0) WT shFABP4 WT shFABP4 HFD SC B LCFA desaturation index (C16:1/C16:0) WT shFABP4 WT shFABP4 HFD SC C SCD1 mRNA levels relative to WT SC controls WT shFABP4 WT shFABP4 HFD SC

6. 2.0 Liver leptin mRNA relative to EWAT 1.5 1.0 ND ND ND ND 0.5 0.0 HFD SC 3 2 2 1 3 1 0.8 1 0 2 0.6 HFD SC 0.4 1 0.2 0 HFD SC 0 0 HFD SC HFD SC 8 6 3 4 30 2 2 20 0 HFD SC 1 10 20 0 15 60 0 HFD SC HFD SC 50 15 10 40 25 5 30 10 20 0 20 HFD SC 15 10 5 10 0 HFD SC 5 0 0 HFD SC HFD SC Figure 5 A p=0.01 Liver FABP4 mRNA relative to WT SC CD68 mRNA relative to WT SC * * p=0.01 F4/80 mRNA relative to WT SC MIP-1 mRNA relative to WT SC ADAM8 mRNA relative to WT SC B * * Leptin mRNA relative to WT SC CD68 mRNA relative to WT SC F4/80 mRNA relative to WT SC * * * MIP-1 mRNA relative to WT SC MAC-1 mRNA relative to WT SC ADAM8 mRNA relative to WT SC