Download
protein folding n.
Skip this Video
Loading SlideShow in 5 Seconds..
Protein Folding PowerPoint Presentation
Download Presentation
Protein Folding

Protein Folding

13 Vues Download Presentation
Télécharger la présentation

Protein Folding

- - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

  1. Protein Folding David Shiuan Department of Life Science, Institute of Biotechnology and Interdisciplinary Program of Bioinformatics National Dong Hwa University

  2. Molecular Forces • Covalent Interaction • Nonbonded Interaction - Electrostatic Interaction - Hydrophobic Interaction - Van der wall Force

  3. PROTEIN FOLDING AND DISEASE • What happens if proteins don't fold correctly? Diseases such as Alzheimer's disease, cystic fibrosis, BSE (Mad Cow disease), an inherited form of emphysema, and even many cancers are believed to result from protein misfolding. • When proteins misfold, they can clump together ("aggregate"). These clumps can often gather in the brain, where they are believed to cause the symptoms of Mad Cow or Alzheimer's disease.

  4. PROTEIN FOLDING AND NANOTECHNOLOGY Building man made machines on the nanoscale • In addition to biomedical applications, learning about how proteins fold will also teach us how to design our own protein-sized "nanomachines" to do similar tasks. Of course, before nanomachines can carry out any activity, they must also be assembled.

  5. Structure Determination • 1. X-ray Diffraction • 2. NMR Spectroscopy • 3. Theoretical Calculation

  6. Protein Structure • Primary Structure • Secondary Structure • Tertiary Structure • Quaternary Structure

  7. Types of Secondary Structure • Helices • Sheets • Turns • Others

  8. Anti-parallel b sheet

  9. HPV virus

  10. Types of Protein • Globular Protein • Membrane Protein • Fibrous and Structural Protein

  11. Globular Protein • Most protein • Proteins > 200 aa have multi-domains

  12. Protein Interactions • 1. Regulatory Protein and DNA • 2. Enzymes • 3. Allostery – hemoglobin • 4. Muscle Fibres

  13. Lambda phage Cro repressor-operator interaction

  14. Insulin hexamer - zinc ions and 3 water coordinate with each

  15. Active Site

  16. Enzyme reaction mechanism

  17. Enzyme reaction mechanism

  18. Allosteric Effect, Cooperative Binding