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Integrated Light Microscopy and Electron Microscopy

Integrated Light Microscopy and Electron Microscopy. R G B is boring. But C M Y K spells FUN!. C yan M agenta Y ellow Blac K. Plain old B / W BSE image, (OPC 24 hours). Prettier when inverted and assigned to Y K. But still kind of boring. Maybe optical microscopy can help!.

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Integrated Light Microscopy and Electron Microscopy

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  1. Integrated Light Microscopy and Electron Microscopy

  2. RGB is boring.

  3. But CMYK spells FUN!

  4. Cyan Magenta Yellow BlacK

  5. Plain old B/W BSE image, (OPC 24 hours).

  6. Prettier when inverted and assigned to YK.

  7. But still kind of boring.

  8. Maybe optical microscopy can help!

  9. Transmitted light.

  10. Crossed-polars.

  11. Epifluorescent mode.

  12. Let’s try transmitted light first.

  13. Take the R-channel.

  14. Return to the YK-BSE image.

  15. Assign transmitted light to C.

  16. Look at all those sky-blue opaque interstitials!

  17. Now it’s crossed-polars’ turn.

  18. Take the B-channel.

  19. Invert it.

  20. Return to the C–transmitted, YK–BSE image.

  21. Assign crossed-polars to M.

  22. CMYK!

  23. Look at all those rosy birefringent portlandites!

  24. From boring old B/W BSE…

  25. …to optically-enhanced CMYK BSE!

  26. In just seven easy steps!

  27. Almost like traditional Chinese water-color!

  28. Kind of a stretch, but at least water-coloresque!

  29. OPC, 24 hours

  30. OPC, 96 hours

  31. OPC, 960 hours

  32. OPC, 24 hours

  33. OPC, 96 hours

  34. OPC, 960 hours

  35. OPC & fly ash, 24 hours

  36. OPC & fly ash, 96 hours

  37. OPC & fly ash, 960 hours

  38. OPC & fly ash, 24 hours

  39. OPC & fly ash, 96 hours

  40. OPC & fly ash, 960 hours

  41. OPC & limestone dust, 24 hours

  42. OPC & limestone dust, 96 hours

  43. OPC & limestone dust, 960 hours

  44. OPC & limestone dust, 24 hours

  45. OPC & limestone dust, 96 hours

  46. OPC & limestone dust, 960 hours

  47. Can I still record optical images after I’ve carbon coated?

  48. Sure, as long as the carbon coating hasn’t cracked up.

  49. Just increase the shutter speed, because everything gets darker.

  50. Transmitted light, pre-coating, 1/10,000 shutter.

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