1. Integrated Light Microscopy and Electron Microscopy

2. RGB is boring.

3. But CMYK spells FUN!

4. Cyan Magenta Yellow BlacK

5. Plain old B/W BSE image, (OPC 24 hours).

6. Prettier when inverted and assigned to YK.

7. But still kind of boring.

8. Maybe optical microscopy can help!

9. Transmitted light.

10. Crossed-polars.

11. Epifluorescent mode.

12. Let’s try transmitted light first.

13. Take the R-channel.

14. Return to the YK-BSE image.

15. Assign transmitted light to C.

16. Look at all those sky-blue opaque interstitials!

17. Now it’s crossed-polars’ turn.

18. Take the B-channel.

19. Invert it.

20. Return to the C–transmitted, YK–BSE image.

21. Assign crossed-polars to M.

22. CMYK!

23. Look at all those rosy birefringent portlandites!

24. From boring old B/W BSE…

25. …to optically-enhanced CMYK BSE!

26. In just seven easy steps!

27. Almost like traditional Chinese water-color!

28. Kind of a stretch, but at least water-coloresque!

29. OPC, 24 hours

30. OPC, 96 hours

31. OPC, 960 hours

32. OPC, 24 hours

33. OPC, 96 hours

34. OPC, 960 hours

35. OPC & fly ash, 24 hours

36. OPC & fly ash, 96 hours

37. OPC & fly ash, 960 hours

38. OPC & fly ash, 24 hours

39. OPC & fly ash, 96 hours

40. OPC & fly ash, 960 hours

41. OPC & limestone dust, 24 hours

42. OPC & limestone dust, 96 hours

43. OPC & limestone dust, 960 hours

44. OPC & limestone dust, 24 hours

45. OPC & limestone dust, 96 hours

46. OPC & limestone dust, 960 hours

47. Can I still record optical images after I’ve carbon coated?

48. Sure, as long as the carbon coating hasn’t cracked up.

49. Just increase the shutter speed, because everything gets darker.

50. Transmitted light, pre-coating, 1/10,000 shutter.