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Supplementary Figure 1

Supplementary Figure 1. A. 827 + EGF/bFGF. 827 + EGF/bFGF. 827 + EGF/bFGF. 0. PHA665752. SU11274. SGX523. -1. Proportion of wells w/o sphere (log 10 ). -2. -3. -4. 0. 50. 100. 150. 200. 0. 10. 20. 30. 40. 50. 0. 5. 10. 15. 20. 827 + HGF. 827 + HGF. 827 + HGF. 0.

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Supplementary Figure 1

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  1. Supplementary Figure 1 A. 827 + EGF/bFGF 827 + EGF/bFGF 827 + EGF/bFGF 0 PHA665752 SU11274 SGX523 -1 Proportion of wells w/o sphere (log10) -2 -3 -4 0 50 100 150 200 0 10 20 30 40 50 0 5 10 15 20 827 + HGF 827 + HGF 827 + HGF 0 PHA665752 SU11274 SGX523 -1 Proportion of wells w/o sphere (log10) -2 -3 -4 0 50 100 150 200 0 20 40 60 80 100 0 20 40 60 80 100 number of plated cells B. 131 + EGF/bFGF 131 + EGF/bFGF 131 + EGF/bFGF 0 PHA665752 SU11274 SGX523 -1 Proportion of wells w/o sphere (log10) -2 -3 -4 0 100 200 300 400 500 20 40 60 80 100 0 0 50 100 150 200 131 + HGF 131 + HGF 131 + HGF 0 PHA665752 SU11274 SGX523 -1 Proportion of wells w/o sphere (log10) -2 -3 -4 0 50 100 150 200 0 50 100 150 200 0 50 100 150 200 number of plated cells

  2. Supplementary Figure 2 464T +EGF/bFGF 464T +EGF/bFGF -SU11274 464T +EGF/bFGF +SU11274 2.08% 31.8% 25.4% CD133-APC Isotype-APC 464T +HGF 464T +HGF -SU11274 464T +HGF +SU11274 Isotype-APC 2.83% CD133-APC 9.00% 7.53%

  3. Supplementary Figure 3 GFP ABC Merge Merge + DAPI Field 1 GFP ABC Merge Merge + DAPI Field 2 GFP ABC Merge Merge + DAPI Field 3

  4. Supplementary Figure 4 mCMV backbone reporter mCMV-TCF/LEF reporter 464T Relative Luciferase Unit Wnt3a (ng/ml) 0 25 50

  5. Supplementary table 1 Expression levels of WNT target genes in METhigh/+ cells

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