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Exploring Naphthalene-Degrading Genes for Bioremediation Applications

Discover small, measurable Output Genes with characteristics suitable for beneficial pharmaceutical, biofuel, and chemical commodities applications. These genes function in E. coli but can be orthogonal. Explore naphthalene degradation through an unoptimized 2-gene operon encompassing NhaC and NhaS, with a potential for blue coloring when oxidized.

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Exploring Naphthalene-Degrading Genes for Bioremediation Applications

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  1. Dream Gene

  2. Characteristics • Small (less than 1,200 base pairs) • Measurable Output (Riboswitch, Color) • Functions in E. coli, but orthogonal • Already Studied/or not • Beneficial • Pharmaceutical • Biofuel • Chemical Commodities • Bioremediation

  3. Websites about Naphthalene • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC106482/pdf/am003633.pdf • http://www.ncbi.nlm.nih.gov/pubmed/16804694 • http://www.ncbi.nlm.nih.gov/pubmed/16923069 • http://www.ncbi.nlm.nih.gov/pubmed/17937670 • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC168525/pdf/632330.pdf • http://www.uniprot.org/uniprot/D5D8A6 • http://jb.asm.org/content/179/12/3851.full.pdf+html

  4. Naphthalene (Na+/H+ antiporterNhaC) Pros Cons A part of the sequence might get stuck between the phospholipids bilayer “2-gene operon encompassing nhaC and a small gene that was designated nhaS” Directly downstream of nt repeats • 1,401 base pairs • In E. coli • Has not been optimized • Active Transport: solute A(out) + solute B(in) = solute A(in) + solute B(out). • Blue coloring when oxidized • 7 different genes to choose from

  5. Website for other genes • http://www.ncbi.nlm.nih.gov/gene

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