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This study presents the characterization of various TAL protein variants, specifically pET-opTAL and pET-TAL, with their respective molecular weights noted. Figures S1 and S2 illustrate the structural differences and functional implications of these proteins, while Table S1 summarizes the results of the Tukey test on the data collected from Figure 3. Additionally, Table S2 lists the oligonucleotide primers utilized throughout this research, providing essential tools for genetic analysis. This comprehensive investigation enhances our understanding of TAL proteins in genetic applications.
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Fig S1 A) B) pET-opTAL pET-TAL M 3 4 kDa kDa M 1 2 95 95 72 72 55 TAL opTAL 55 43 43 * 34 34
Fig S2 A) B) pET-opT5 pET-T5 M 3 4 M 1 2 kDa kDa 95 95 72 72 Sam5(59kDa) Sam5 (59kDa) 55 TAL (57.4kDa) 55 opTAL (56kDa) 43 43 34 34
Table S1The results of the Tukey test for the data from Figure 3