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This study analyzes the regulation of apoptosis-related proteins in human primary cardiac myocytes exposed to APOSECH using membrane array analysis. The results show up-regulation of Bcl-2 and Heat shock proteins. The methods utilized Human Apoptosis Antibody Array Ary009 from R&D Systems, USA, following the manufacturer's protocol.
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ApoptosisArray 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A B C D E Medium A B C D E APOSEC
Supplementary Figure 3: Membrane array analysis for apoptosis related proteins regulated by APOSECH in human primaray cardiac myocytes. As shown, mainly Bcl-2 and Heat shock proteins were up-regulated after exposure to APOSECH. Supplementary Figure 3 Methods Membrane arrays for the detection of factors that regulate apoptosis in human primary cardiac myocytes that were exposed to APOSECH (Human Apoptosis Antibody Array Ary009, R&D Systems, USA) were utilized. Experiments were performed according to the manufacturer’s protocol.