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Supplemental Fig. 7. Telomeric position effects are relieved by histone deacetylation inhibitors.

A. Percent of cells expressing dsRed and GFP. B. untreated. TSA. NaB. 5-Aza. BrdU. Percent of cells expressing dsRed and GFP. Supplemental Fig. 7. Telomeric position effects are relieved by histone deacetylation inhibitors.

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Supplemental Fig. 7. Telomeric position effects are relieved by histone deacetylation inhibitors.

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  1. A Percent of cells expressing dsRed and GFP B untreated TSA NaB 5-Aza BrdU Percent of cells expressing dsRed and GFP Supplemental Fig. 7. Telomeric position effects are relieved by histone deacetylation inhibitors. The four clones with telomeric integration displaying low (A) or significant transgene expression levels (B) were treated with TSA (1µM), Na butyrate (NaB, 1mM), 5azadC (3µM) or BrdU (50µM). Loss of silencing effects were assessed by recording the percentile of cells showing an increase of GFP and DsRed fluorescence as compared to the untreated cell populations shown in Fig S1. Both HDAC inhibitors (TSA and NaB) and BrdU treatment relieved transgene silencing as compared to the untreated sample whereas DNA methylation inhibitor (5azadC) had no effect.

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