1 / 10

% survival

Sensitivity to H 2 0 2 and Glutathione Levels. 100. 100. 80. 80. 60. 60. % GSH content. % survival. 40. 40. 20. 20. 0. 0. w.t. D ynk. w.t. D ynk. NDP kinase Activity. Autophosphorylation Activity. Structure and Functional Analysis of YNK. 100. 80. 60. % survival. 40.

maura
Télécharger la présentation

% survival

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Sensitivity to H202 and Glutathione Levels 100 100 80 80 60 60 % GSH content % survival 40 40 20 20 0 0 w.t. Dynk w.t. Dynk

  2. NDP kinase Activity Autophosphorylation Activity

  3. Structure and Functional Analysis of YNK 100 80 60 % survival 40 20 0 w.t. Dynk Dynk + YNK Dynk + H119F Dynk + P73H Dynk + C118A

  4. Proteomix Analysis ofynk1 Knock-Out w.t. Dynk w.t. + H202 Dynk + H202 CPY: PPIase

  5. Arabidopsis

  6. Accumulation of NDPK2 Transcript during Oxidative Stress

  7. ATP GTP Enhanced NDP Kinase and Autophosphorylation Activity in Transgenic Plant Overexpressing NDPK2 transgenic w.t. transgenic w.t. H2O2 : 0 5 10 0 5 10 (mM) 0 5 10 20 30 0 5 10 20 30 (mM) NDPK activity autophosphorylation activity

  8. NDPK2 Regulates Cellular Redox Conditions wild-type transgenic knock-out 100 relative fluorescence (%) 50 0 w. t. k. o. t. g.

  9. NDPK2 Determines Phosphorylation of Two Putative Oxidative Stress Activated Proteins wild-type + H202 transgenic knock-out wild-type 47 kD 42 kD

More Related