1 / 6

Understanding Volume Measurement: A Comprehensive Guide to Instruments and Techniques

This chapter explores the definition of volume, the basic units of measurement, and the various instruments used to measure volume in laboratory settings. It covers the use of graduated cylinders, volumetric flasks, and micropipettors, addressing their accuracy, calibration methods (TC or TD), and specific applications. Micropipetting techniques are discussed, focusing on positive and air displacement methods, and their effectiveness with different solutions. The chapter concludes with a comparison of instruments and validation processes, making it essential for anyone involved in scientific measurements.

oakes
Télécharger la présentation

Understanding Volume Measurement: A Comprehensive Guide to Instruments and Techniques

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chapter 16

  2. Chapter 16 • In General…… • What is the definition of volume and what is the basic unit? • How do you use a graduate cylinder to measure volume? • What are the instruments used to measure volume? • In order of increasing accuracy, list the instruments that are used to measure volume. • How are micropipettors validated?

  3. Glassware • Calibrated either TC or TD-what does this mean? • What are the tolerances for a volumetric in comparison to a graduated cylinder? • Compare and contrast the use of volumetrics, graduated cylinders, beakers and flasks • What is a serological pipet? • What is a Mohr pipet? • What is a Pasteur pipet?

  4. Micropipetting Devices • Positive displacement vs air displacement

  5. Pipetting of Difficult Solutions • Air displacement micropipettors are not very accurate when dispensing volatile liquids (like acetone) or viscous materials (like protein solutions). • Review Table 16.3

  6. Validation of Micropipettors • Box 5 pages 314-315 • Problems • All

More Related