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Part 2

Part 2. Transcription 1. Transcription 2. Translation 1. Translation 2. Mutations. 100. 100. 100. 100. 100. 200. 200. 200. 200. 200. 300. 300. 300. 300. 300. 400. 400. 400. 400. 400. 500. 500. 500. 500. 500.

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Part 2

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Presentation Transcript

  1. Part 2

  2. Transcription 1 Transcription 2 Translation 1 Translation 2 Mutations 100 100 100 100 100 200 200 200 200 200 300 300 300 300 300 400 400 400 400 400 500 500 500 500 500

  3. Where does transcription take place? (name part of eukaryotic cell)What are copied from DNA during transcription?

  4. In the nucleus!Produces RNA (mRNA, tRNA, rRNA)Transcription #1 100

  5. What are the sites on Prokaryotes called that regulate gene expression during transcription? Why is gene regulation important in Eukaryotes?

  6. Regulatory Sites Regulatory Sites Without these gene expression could not be controlled (turn on or off) in Prokaryotes Gene regulation in Eukaryotes allows for cell specialization Transcription #1 200

  7. What is the function of the promoter?

  8. Binding site of the RNA Polymerase to begin making mRNA Transcription #1 300

  9. An ______________is a group of genes that work together to regulate transcription in prokaryotes

  10. OperonTranscription #1 400

  11. How does the lac repressor turn off the lac genes in the lac operon?

  12. By binding to the operator and blocking transcription by RNA Polymerase Transcription #1 500

  13. Transcribe this DNA strand into mRNA…T A C A C G C A G T C A (DNA) mRNA

  14. T A C A C G C A G A T T (DNA)A U G U G C G U C U A A(mRNA)Each codon has 3 nucleotides eachTranscription #2 100

  15. What is the entire process of transcription and translation called?

  16. Protein SynthesisTranscription #2 200

  17. When an mRNA is being transcribed, what is edited out and what is kept in?

  18. Introns Out! “junk” Transcription #2 300 Exons In!

  19. What is this object leaving the nucleus after transcription?

  20. Transcription #2 400 mRNA

  21. What is the part of a Eukaryote’s DNA that signals where the RNA polymerase is to begin transcribing? Hint:

  22. The TATA Box is the promoter region in eukaryotes for transcription. Transcription #2 500

  23. Where does the mRNA go for translation to occur?

  24. Out of the nucleus and into the cytoplasm to the ribosomeTranslation #1 100 Cytoplasm

  25. Once the mRNA is in the cytoplasm, what RNA recognizes and wraps around the mRNA?

  26. rRNA (ribosomal RNA)Translation #1 200

  27. Where does the ribosome (rRNA) begin reading the mRNA? What amino acid does this code for?

  28. Begins at the “Start” codon AUG (Remember: It’s the month we start school) AUG codes for Methionine (Met) Translation #1 300

  29. What happens to the amino acids that are brought to the ribosome by the tRNA’s during translation? Amino Acid

  30. Amino Acid The amino acid joins the growing polypeptide chain (protein). The tRNA then leaves to find another amino acid. Translation #1 400

  31. When do tRNA’s stop bringing amino acids to the ribosome during translation?

  32. When a STOP codon is reached on the mRNA strand.Translation #1 500 Stop codon Polypeptide chain released into cytoplasm and the ribosome falls off of the mRNA which is disposed of by the cell. Translation #1 500

  33. Translate this mRNA A U G U G C G U C U A A

  34. mRNA A U G U G C G U C U A AAmino Acids Met Cys Val Stop This is TRANSLATION!!! Translation #2 100

  35. What does this wheel show? What process is taking place when you use this wheel?

  36. Translation #2 200 Shows the GENETIC CODEThe process is TRANSLATION

  37. What is the max possible number of codons for a single amino acid? How many codons specify for the amino acid Threonine? What are they?

  38. Threonine ACG ACA ACCACU 6 is the maximum number or codons possible for an amino acid! Translation #2 300

  39. What brings the amino acid to the ribosome to make proteins during translation?

  40. tRNA brings 1 Amino Acid Translation #2 400

  41. How many different codons are there?How many different amino acids are there?

  42. Different codons Different amino acids Translation #2 500

  43. Not a real picture What is a gene mutation?

  44. Change in nucleotide sequenceMutations for 100

  45. Of the following mutations, which one of these is not a gene mutation?SubstitutionDeletion InsertionInversion Gene mutation in mice.

  46. Substitution = Point Mutation (gene)Deletion = Frame Shift Mutation (gene)Insertion = Frame Shift Mutation (gene) Inversion = Chromosomal Mutation I bet he can catch 3x more flies! Mutations for 200

  47. What type of gene mutation is it when a nucleotide is inserted or deleted?

  48. The amino acid sequence is no longer the same because the codons have shifted Frame Shift Gene Mutation Mutations for 300

  49. Name all types of chromosomal mutations

  50. Mutations for 400

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