1 / 13

Gd-DTPA as an MRI Contrast Agent

Gd-DTPA as an MRI Contrast Agent. Lindsay Dods April 6, 2010 Supervisors: Robert Ta, Masters Student Robert Bartha, Ph.D. Introduction. MRI – Magnetic Resonance Imaging Can be used to detect anatomical structures and function. Contrast Agents

rlindsey
Télécharger la présentation

Gd-DTPA as an MRI Contrast Agent

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Gd-DTPA as an MRI Contrast Agent Lindsay Dods April 6, 2010 Supervisors: Robert Ta, Masters Student Robert Bartha, Ph.D.

  2. Introduction • MRI – Magnetic Resonance Imaging • Can be used to detect anatomical structures and function. • Contrast Agents • Can enhance the contrast of an MRI image, allowing for increased differentiation. • Novel Contrast Agents must be tested

  3. Objectives • Show the effectiveness of Gd-DTPA (Magnevist) as a contrast agent in high field MRI. • As this agent is known to be effective, the true objective is to assess the testing protocol before using novel contrast agents.

  4. Approach • Compare contrast in mice brains • before and after incubation in contrast agent • different incubation times. • Contrast is ratio of mean signal intensity of the brain compared to control

  5. Hypothesis • The mean signal intensity of the brain will increase with the addition of contrast agent • This signal intensity will continue to increase over time, until a saturation point is reached • Intensity of the control is to remain constant.

  6. Methods • Prepare Samples • Split fixed brain into two hemispheres • Incubate one side of the brain in diluted Gd-DTPA, use other side as control

  7. Methods • Scan • Perform T1 and T2 waited scans with a 9.4 T MRI. • Measure mean grey level of each hemisphere from select slices in ImageJ • Calculate Contrast

  8. Day 1, T2 Day 6, T2 Results

  9. Results • Using Regression Analysis, P<0.05, therefore result is significant.

  10. Results • Using Regression Analysis, P<0.05, therefore result is significant.

  11. Discussion • Results aren’t as expected • Paramagnetic contrast agents alter signal intensity by shortening relaxation time of protons • Increase in signal intensity is beneficial

  12. Future Work • Contrast agent in disease diagnosis • Use this protocol on novel contrast agents

  13. Conclusion • Results were not conclusive to show that signal intensity increased over time with the addition of a contrast agent. • More samples are needed to determine if this protocol is an effective way to test contrast agents.

More Related