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# Repeated Measures Designs

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1. Repeated Measures Designs

2. In a Repeated Measures Design We have experimental units that • may be grouped according to one or several factors (the grouping factors) Then on each experimental unit we have • not a single measurement but a group of measurements (the repeated measures) • The repeated measures may be taken at combinations of levels of one or several factors (The repeated measures factors)

3. Example In the following study the experimenter was interested in how the level of a certain enzyme changed in cardiac patients after open heart surgery. • The enzyme was measured • immediately after surgery (Day 0), • one day (Day 1), • two days (Day 2) and • one week (Day 7) after surgery • for n = 15 cardiac surgical patients.

4. The data is given in the table below. Table: The enzyme levels -immediately after surgery (Day 0), one day (Day 1),two days (Day 2) and one week (Day 7) after surgery

5. The subjects are not grouped (single group). • There is one repeated measures factor -Time – with levels • Day 0, • Day 1, • Day 2, • Day 7 • This design is the same as a randomized block design with • Blocks = subjects

6. The Anova Model for a simple repeated measures design Repeated measures y11 y12 y13 … y1t y21 y22 y23 … y2t subjects yn1 yn2 y13 … ynt

7. The Model yij = the jth repeated measure on the ithsubject = m + ai + tj + eij where m = the mean effect, ai = the effect of subject i, tj = the effect of time j, eij = random error.

8. The Anova Table for Enzyme Experiment The Subject Source of variability is modelling the variability between subjects The ERROR Source of variability is modelling the variability within subjects

9. The repeated measures are in columns Analysis Using SPSS- the data file

10. Select General Linear model -> Repeated Measures

11. Specify the variables that represent the levels of the repeated measures factor There is no Between subject factor in this example

12. The ANOVA table

13. The Multivariate Model for a Repeated measures design

14. The Anova (univariate) Model yij = the jth repeated measure on the ithsubject = m + aj + tj + eij where m = the mean effect, aj = the effect of subject i, tj = the effect of time j, eij = random error.

15. Implications of The Anova (univariate) Model mj = the mean of y ij = m + 0 + tj + 0 = m + tj

16. The implication of the ANOVA model for a repeated measures design is that the correlation between repeated measures is constant.

17. The multivariate model for a repeated measures design Let denote a sample of n from the p-variate normal distribution with mean vector and covariance matrix S. Here Allows for arbitrary correlation structure amongst the repeated measures – yi1, yi2, … , yit

18. Test for equality of repeated measures

19. Repeated measures equal X … t 1 2 3 repeated measures

20. Let Then

21. The test for equality of repeated measures: Consider the data This is a sample of n from the (t – 1)-variate normal distribution with mean vector and covariance matrix .

22. Hotelling’s T2 test for equality of variables if H0 is true than has an F distribution with n1= t – 1 and n2= n - t + 1 Thus we reject H0 if F > Fawith n1= p – 1 and n2= n – t + 1

23. To perform the test, compute differences of successive variables for each case in the group and perform the one-sample Hotelling’s T2 test for a zero mean vector

24. Example

25. Example In the following study the experimenter was interested in how the level of a certain enzyme changed in cardiac patients after open heart surgery. • The enzyme was measured • immediately after surgery (Day 0), • one day (Day 1), • two days (Day 2) and • one week (Day 7) after surgery • for n = 15 cardiac surgical patients.

26. The data is given in the table below. Table: The enzyme levels -immediately after surgery (Day 0), one day (Day 1),two days (Day 2) and one week (Day 7) after surgery

27. Example:(Repeated Measures Design - Grouping Factor) • In the following study, similar to example 3, the experimenter was interested in how the level of a certain enzyme changed in cardiac patients after open heart surgery. • In addition the experimenter was interested in how two drug treatments (A and B) would also effect the level of the enzyme.

28. The 24 patients were randomly divided into three groups of n= 8 patients. • The first group of patients were left untreated as a control group while • the second and third group were given drug treatments A and B respectively. • Again the enzyme was measured immediately after surgery (Day 0), one day (Day 1), two days (Day 2) and one week (Day 7) after surgery for each of the cardiac surgical patients in the study.

29. Table: The enzyme levels - immediately after surgery (Day 0), one day (Day 1),two days (Day 2) and one week (Day 7) after surgeryfor three treatment groups (control, Drug A, Drug B)

30. The subjects are grouped by treatment • control, • Drug A, • Drug B • There is one repeated measures factor -Time – with levels • Day 0, • Day 1, • Day 2, • Day 7

31. The Model yikj = the jth repeated measure on the ithsubject in the kth group = m + ak +ekj (1)+ tj+ (at)ki + ekij(2) where m = the mean effect, ak = the effect of group i, eij(1)= between subject error. tj = the effect of time j,

32. (at)kj = the group-time interaction effect eij(2)= within subject error.

33. The Anova Table There are two sources of Error in a repeated measures design: The betweensubject error – Error1 and the withinsubject error – Error2

34. Tables of means Drug Day 0 Day 1 Day 2 Day 7 Overall Control 118.63 77.88 60.50 55.75 78.19 A 103.25 68.25 52.00 51.50 68.75 B 103.38 69.38 54.13 51.50 69.59 Overall 108.42 71.83 55.54 52.92 72.18

35. The Multivariate approach to this data: • k = 3 samples from 4-variate normal distribution

36. Analysis

37. Example: Repeated Measures Design - Two Grouping Factors • In the following example , the researcher was interested in how the levels of Anxiety (high and low) and Tension (none and high) affected error rates in performing a specified task. • In addition the researcher was interested in how the error rates also changed over time. • Four groups of three subjects diagnosed in the four Anxiety-Tension categories were asked to perform the task at four different times patients in the study.

38. The Anova Table