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This resource presents a comprehensive set of histology slides for the gastrointestinal (GI) tract, organized by magnification levels (40X, 100X, and 400X). As you explore the slides, you are encouraged to identify the organ from which each tissue sample is derived and the specific structures or layers highlighted. Key tissues include esophageal, gastric, liver, pancreas, small intestine, and large intestine samples. Answers to identification goals will be provided at the end of the slide sequence to reinforce learning.
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Histology Slides for the GI Tract • Slides are presented in order of magnification • As you view the following slides make sure you can accomplish these goals: • Can you identify the organ from which the tissue sample was taken? • Can you identify the specific structures or layers indicated by the numbered arrows or brackets? • At the end of a sequence you will find the answers to the above for each organ.
Organ? 40X 3 1 2 4 5 6 7
Organ? 100X 1 2 3 4 5
Organ ? 100X 1 4 2 3
Organ? 400X 1 2 3 4
Tissue from Esophagus slides 3-6 Slide # 3 • Nonkeratinized Stratified Squamous • Lamina Propria • General layer called the Mucosa • Muscularis Mucosae • Submucosal Mucous Gland • Submucosa • Circular smooth muscle layer • Slide # 4 • Nonkeratinized Stratified Squamous • Lamina Propria • Muscularis Mucosae • Submucosal Mucous Gland • Submucosa
Tissue from Esophagus slides 3-6 Slide # 5 • Circular smooth muscle layer • Longitudinal smooth muscle layer • Adventitia • Mucularis Externa • Slide # 6 • Nonkeratinized Stratified Squamous • Lamina Propria • Muscularis Mucosae • Submucosal Mucous Gland
Organ or Organs? 100X 1 2 1 3 4
Slides 9-10 Esophageal-Gastric Junction Slide # 9 • Junction where epithelia changes from nonkeratinized stratified squamous to simple columnar. • Simple columnar • Nonkeratinized stratified squamous • Lumen • Slide # 10 • Simple columnar lining a gastric pit • Junction where epithelia changes from nonkeratinized • stratified squamous to simple columnar
Organ? 100X 1 2 4 3 5 6
Organ? 400X 1 2 3 4 5
Organ? 400X 9 8 2 7 3 1 4 5 6
Slides 12-15 Stomach Slide # 12 Large fold is a Rugae • Gastric Mucosa • Slide # 13 • Simple columnar lining a gastric pit • Muscularis Mucosae • Lamina Propria • Oblique smooth muscle layer • Circular smooth muscle layer • Longitudinal smooth muscle layer
Slides 12-15 Stomach Slide # 14 • Muscularis Mucosae • Submucosa • Oblique smooth muscle layer • Circular smooth muscle layer • Longitudinal smooth muscle layer • Slide # 15 • Muscularis Mucosae • G-cells at the bottom of a gastric pit • Submucosa • Lamina Propria • Parietal cells in a gastric pit • Chief cells in a gastric pit • Oblique smooth muscle layer • Circular smooth muscle layer • Longitudinal smooth muscle layer
Organ? 40X 1 2 2
Organ? 400X 2 1 3
Organ? 400X 2 3 4 1
Slides 18-20 Liver Tissue Slide # 18 • Central Vein • Portal triads demonstrating the boundary of the lobule • Slide # 19 • Central Vein • Sinusoids • Hepatocyte • Slide # 20 • Branch of the Hepatic Portal Vein • Bile duct • Branch of the Hepatic Artery • Portal Triad
Organ? 40X 2 1
Organ? 100X 1 3 2
Organ? 100X 1 2
Slides 22-24 Pancreas Tissue Slide # 22 • Block of acinar cells called an acini • Pacinian Corpuscle • Slide # 23 • Block of acinar cells called an acini • Pacinian Corpuscle • Islet of Langerhans • Slide # 24 • Acinar Cell • Islet of Langerhans
Organ and folds? 40X 9 2 1 4 3 5 6 7 8
Organ? 100X 1 6 2 3 5 4
Organ? 400X 1 2 3 4 5
Slides 26-28 Small Intestine - Duodenum Slide # 26 • Villus • Lamina Propria • Lacteal • Crypt of Lieberkuhn • Submucosa • Visceral Peritoneum • Longitudinal smooth muscle layer • Circular smooth muscle layer • Plicae Circularis
Slides 26-28 Small Intestine - Duodenum Slide # 27 • Villus • Lamina Propria • Muscularis Mucosae • Brunner’s gland • Submucosa • Crypt of Lieberkuhn • Slide # 28 • Villus • Lamina Propria • Goblet cells with mucous drop • Crypt of Lieberkuhn • Muscularis Mucosae
Organ? 100X 1 2 4 6 5 3
Organ? 400X 1 2
Slides 31-32 Small Intestine - Ileum Slide # 31 • Villus • Peyer’s Patch • Muscularis Mucosae • Submucosa • Longitudinal smooth muscle layer • Circular smooth muscle layer • Slide # 32 • Muscularis Mucosae • Peyer’s Patch
Organ? 40X 1 2 3 5 4 6
Organ? 100X 1 2 3 5 4
Organ? 400X 1 3 2 4
Slides 34-36 Large Intestine Slide # 34 • Intestinal Mucosa with intestinal pits or gland • Muscularis Mucosae • Submucosa • Longitudinal smooth muscle layer • Circular smooth muscle layer • Lymph node • Slide # 35 • Intestinal pit or gland • Muscularis Mucosae • Submucosa • Longitudinal smooth muscle layer • Circular smooth muscle layer
Slides 34-36 Large Intestine Slide # 36 • Intestinal pits with goblet cells • Lamina Propria • Muscularis Mucosae • Lumen of an intestinal pit or gland
Organ or organs? 100X 3 2 1 4
Slides 39-41 Ano-rectal junction • Slide # 40 • Junction of the Rectum and Anus • Simple Columnar cells of the Rectum • Nonkeratinized stratified squamous of the Anus • Intestinal pit or gland Slide # 39 • Junction of the Rectum and Anus • Slide # 41 • Simple Columnar cells of the Rectum • Nonkeratinized stratified squamous of the Anus