Supplementary Figure 1

1. pbNK CD56 CD3 dNK CD56 CD3 Supplementary Figure 1 A B

2. Notch 1 Notch 2 Notch 3 Notch 4 Primary NK Notch 1 Notch 2 500 400 Notch 1 Notch 2 Notch 3 Notch 4 YTS 400 500 500 400 dNK C D

3. 6.6 6.9 6.4 4.7 4.7 4.9 15.8 16.6 13.3 Supplementary Figure 2 + IL-15 NO CYTOKINES + IL-12 Primary NK YTS JURKAT

4. Supplementary Figure 3 Delta4-Ig A B Delta1-Ig Primary NK Primary NK YTS YTS NK92 NK-92 Jurkat Jurkat 0.5 μg 5 μg 2.5 μg 1 μg 0.1 μg 0.5 μg 5 μg 2.5 μg 1 μg 0.1 μg C Jagged1-Ig Primary NK YTS NK92 Jurkat 5g 2.5g 1g 0.5g 0.1 μg