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Timing and Location of the Elastic Extracellular Matrix Protein MAGP During Embryonic Development

Timing and Location of the Elastic Extracellular Matrix Protein MAGP During Embryonic Development. Justin Harper and Barbara Trask Ph.D.

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Timing and Location of the Elastic Extracellular Matrix Protein MAGP During Embryonic Development

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  1. Timing and Location of the Elastic Extracellular Matrix Protein MAGP During Embryonic Development Justin Harper and Barbara Trask Ph.D.

  2. Ex tra cell u lar ma trix (eks tre sel u lar ma triks) n. Intercellular substance synthesized by cells and in which cells are embedded; comprised of a variable combination of molecules including proteins, glycoproteins and proteoglycans. Precise composition is dependent upon the structure and function of the tissue in which it is found. e.g., collagenous matrices, elastic matrices, etc. aka: connective tissue.

  3. The ECM Performs Multiple Functions Motility Structure Molecular Reservoir Adhesion Mecham and Davis, 1994. in: Extracellular Matrix Assembly and Structure.

  4. Function of the Elastic Extracellular Matrix • Elastic Matrices function in tissues where repeated cycles of extension and recoil are required. i.e. Lungs, Aorta, etc.

  5. The Function of Elastin

  6. Components of Elastic Extracellular Matrices • Elastin • Microfibrils • Fibrillins • Fibulins • Type VI Collagen • MAGP *The function of each microfibrillar protein in respect to this matrix is still undetermined

  7. Diseases of the EEM • Disfunctional EEMs are linked with conditions such as Emphysema, Marfan Syndrome, and Abdominal Aortic Aneurisms.

  8. Model OrganismZebrafish (Danio rerio)

  9. Ventral Aorta CiliaryZonules Elastic Matrices and Microfibrils in Zebrafish

  10. Discovering MAGP in Zebrafish • We used the described sequence of MAGP from Xenopus laevis (African clawed frog) to engineer primers for MAGP in Danio rerio (Zebrafish) • C primers • F Primers

  11. Conservation Between Danio rerio and Xenopus laevis Amino Acid Sequences

  12. Optimization of PCR

  13. 8Somite 21Somite 26 hpf 42 hpf 72 hpf Embryonic Stages of Development

  14. Embryonic MAGP Expression

  15. ~1.5 kb An Antisense Probe Recognizes a Single mRNA in 24 Hour Danio rerio Embryos

  16. Subsequent Steps • Expand the Number of Time Points • Both before 8 somite and after 72hpf • In Situ Hybridization • Optimization of the probe concentration and development time

  17. Acknowledgements • Paul Garrett • Elaine Davis Ph.D., McGill University • Shawn Tolbert and Jeff Pack • Weber State University Office of Undergraduate Research • WSU Research Scholarship and Professional Growth Office • Department of Zoology, Weber State University

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