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New Functions For Plant PR-5 (Thaumatin-like) Proteins PowerPoint Presentation
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New Functions For Plant PR-5 (Thaumatin-like) Proteins

New Functions For Plant PR-5 (Thaumatin-like) Proteins

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New Functions For Plant PR-5 (Thaumatin-like) Proteins

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  1. New Functions For Plant PR-5 (Thaumatin-like) Proteins

  2. Plant PR-5 Protein Facts • Ubiquitous Osmotin mRNA per 10 ug total RNA La Rosa et al., (1992) Plant Physiol. 100:409

  3. C * * * * * N * * * * * * * * * * * * * * * * C * * * N PR-5 Proteins Have Conserved Structure Thaumatin, Osmotin TLPs, OLPs (De Vos et al., 1995) (PDB) (Koiwa et al., 1997)

  4. Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. ‘Samsun’ and ‘Samsun NN’ II. Changes in protein constitution after infection with tobacco mosaic virus L. C. Van Loon and A. Van Kammen Laboratory of Virology, State Agricultural University, Wageningen, The Netherlands Accepted 14 August 1969.  Available online 16 April 2004. Abstract Electrophoresis in 5, 7.5, and 10% acrylamide gels revealed that characteristic changes appear in the protein constitution of N. tabacum var. ‘Samsun,’ ‘Samsun NN,’ and N. glutinosa after infection with tobacco mosaic virus (TMV). Apart from significant quantitative changes, one new band was present in Samsun plants 4 weeks after infection. This band was characterized as TMV coat protein by coelectrophoresis and serology. A change in the electrophoretic mobility of the major band was recorded. In Samsun NN plants four new bands (I–IV) were present 1 week after infection. These bands are not related to TMV coat protein and are not new isozymes of peroxidase, polyphenoloxidase, acid phosphatase, glucose-6-phosphate dehydrogenase, or 6-phosphogluconate dehydrogenase. The bands first appear at the onset of necrosis and increase in intensity with time. Five days after infection band I ceases to increase, whereas bands II, III, and IV increase up to day 14. From day 7 onward, the four bands are also present in the noninoculated, young developing leaves, where they further increase with time. The amount of the four new protein components was related to lesion density and was reduced by treatment of the leaves with actinomycin D 2 days after infection. In N. glutinosa one new band was induced and two bands increased markedly after infection, while one had disappeared from the protein pattern. These changes relate to other protein components than those found in infected Samsun NN, but are also apparent in the noninoculated parts of the plants from day 7 after infection. The possible relation of the new protein components to the phenomenon of systemic acquired resistance is discussed.

  5. Signal Transduction RNAse PR-10 Other PR-7 RIP PR-5 Proteins Are Plant Defense Effectors Pathogen Perception Defense genes encoding antimicrobials PR-1s PR-2s PR-5s Chitinases PR-3,-4, -8, -11 Proteinase inhibitors PR-6 In Fond Memory Pathogen Defensins PR-12 Thionins PR-13 LTPs PR-14

  6. Transgenic Overexpression Of PR-5 Genes Increases Stress Tolerance

  7. Arabidopsis thaliana TLPs: characteristics and tissue-specific expression. Adv Bot Res (2009) 17: 439-489.

  8. Induction Of Arabidopsis TLPs By By Stress, Senescence And Nutrients Adv Bot Res (2009) 17: 439-489.

  9. The PR-5 Protein Gene Family In A Plant Species Is Fairly Large Adv Bot Res (2009) 17: 439-489.

  10. Occurence of PR-5/Thaumatin-like Proteins Species • Sequence and structural similarity with plant PR-5 proteins • As families Adv Bot Res (2009) 17: 439-489.