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Effects of siRNA Knockdown on Abl Protein Levels in MDA-MB-435s and BT-549 Cells

This study analyzes the impact of various siRNA treatments on the Abl protein levels in MDA-MB-435s and BT-549 cell lines. Supplementary Figures 1 and 2 demonstrate the knockdown efficiency of Abl-specific siRNAs, showing reductions of 81% and 90%, respectively. In contrast, Supplementary Figure 3 evaluates the effects of Abl knockdown following starvation conditions in BT-549 cells, revealing varied levels of Abl protein. Additionally, α-tubulin blots serve as loading controls, confirming the validity of results across conditions.

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Effects of siRNA Knockdown on Abl Protein Levels in MDA-MB-435s and BT-549 Cells

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  1. Srinivasan, Supplementary Figure 1 MDA-MB-435s IP: Abl Arg scrambled scrambled siRNA: Abl Arg Arg Abl GST-Crk %knockdown: 81 35 0 60 Abl Blot

  2. Srinivasan, Supplementary Figure 2 MDA-MB-435s IP: Abl Arg scrambled scrambled Arg Abl Arg Abl si-RNA: GST-Crk %Knockdown: 90 27 42 86 Abl Blot -tubulin Blot

  3. Srinivasan, Supplementary Figure 3 BT-549 Days post starvation: 3 4 IP: Abl Arg Abl Arg Abl Arg Arg Abl Abl scrambled scrambled Arg scrambled Abl scrambled Arg si-RNA: GST-Crk % Knockdown: 69 0 0 69 62 19 28 77 pan Abl Blots

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