Impact of TP53 Knockout on IC50 of 5-Fluorouracil and Oxaliplatin in HCT116 Cells

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This study investigates the effect of TP53 knockout (HCT116-TP53KO) on the IC50 values of 5-fluorouracil (5FU) and oxaliplatin in HCT116 colorectal cancer cells. Through comparative analysis, we evaluate the sensitivity of HCT116 with and without TP53, assessing cell viability following treatment with varying concentrations of 5FU and oxaliplatin. Results illustrate significant changes in drug responsiveness, highlighting the role of TP53 in mediating chemosensitivity in colorectal cancer.

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May 13, 2026

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Impact of TP53 Knockout on IC50 of 5-Fluorouracil and Oxaliplatin in HCT116 Cells

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HCT116 HCT116-TP53KO NT 5FU Ox NT 5FU Ox 5 2 4 LC3 3 IC50 5FU (µM) 1 IC50 Oxaliplatin (µM) 2 1 GAPDH 0 0 HCT116 HCT116 TP53KO HCT116 HCT116 TP53KO 6 5 4 7 HCT116 IC50 5FU (µM) 3 6 HCT116 0.4 2 5 4 1 0.3 IC50 5FU (µM) 3 IC50 Oxaliplatin (µM) 0 0.2 1.6 siLUC siATG5 si ATG7 2 1.4 1 0.1 1.2 0 1 0 siLUC siATG5 si ATG7 IC50 Oxaliplatin (µM) 0.8 siLUC si ATG5 si ATG7 0.6 0.4 0.2 0 siLUC si ATG5 si ATG7 HCT116-TP53KO HCT116-TP53KO Supplementary Figure 3 A B D C E F