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Neutralization of p300 Prevents E2F1 Accumulation and Apoptosis Induced by Cisplatin in H358 Cells

In this study, we explored the role of p300 in E2F1 accumulation and apoptosis in H358 cells exposed to cisplatin. Cells were transfected with either control (Ctl) or p300 siRNA for 48 hours, followed by treatment with 50 µM cisplatin for an additional 24 hours. (A) Western blotting showed E2F1 and p300 levels, with actin as a loading control. (B) Apoptosis was assessed through Active Caspase-3 staining and flow cytometry analysis. Our findings indicate that p300 neutralization significantly reduces E2F1 accumulation and apoptosis in response to cisplatin.

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Neutralization of p300 Prevents E2F1 Accumulation and Apoptosis Induced by Cisplatin in H358 Cells

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  1. Ctl sip300 p300 E2F1 Actin Cisp (24h) Supplementary Figure 3 A B 25 NT Cisp (24h) 20 Apoptosis (%) 15 10 5 0 Ctl sip300 Neutralization of p300 prevents E2F1 accumulation and apoptosis in response to cisplatin. H358 cells were transfected for 48 hours with either control (Ctl) or p300 siRNA and exposed to 50M cisplatin for an additional 24 hours. (A) Western blotting was performed using anti-E2F1 or anti-p300 antibody. Actin was used as a loading control. (B) Apoptosis was studied by Active Caspase 3 staining followed by FACS analysis.

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