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Comparative Analysis of ANT Contribution to Proton Leak in Muscle and Liver Mitochondria

This study investigates the role of ANT in proton leak in muscle and liver mitochondria from WT and Grx2-/- mice, along with UCP3 protein levels using immunoblot analysis. Results provide insights into mitochondrial function and proton leak regulation.

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Comparative Analysis of ANT Contribution to Proton Leak in Muscle and Liver Mitochondria

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  1. Supplemental Figure 5 p<0.05 p=0.05 a b p<0.05 p<0.05 p<0.05 p<0.05 c Muscle d Liver + - + - UCP3 p<0.01 UCP3 SDH p<0.05 Figure S5: A) Contribution of ANT to proton leak in liver mitochondria from WT and Grx2-/- mice. Following a measure of state 3 respiration, mitochondria were sequentially treated with oligomycin (Oligo; 2.5 μg/mL), carboxyatractyloside (CAT; 5 μM), and antimycin A (4 μM). Oxygen consumption rate (OCR) values were corrected for background (antimycin A) respiration. n=4, mean±SEM, 2-way ANOVA with Fisher’s posthoc test. B) Contribution of ANT to proton leak in muscle mitochondria from WT and Grx2-/- mice. Following a measure of state 3 respiration, mitochondria were sequentially treated with oligomycin (Oligo; 2.5 μg/mL), carboxyatractyloside (CAT; 5 μM), and antimycin A (4 μM). OCR values were corrected for respiration not associated with the electron transport chain (antimycin A). C) Immunoblot analysis of UCP3 protein levels in mitochondria collected from WT and UCP3-/- mice. D) Contribution of ANT to proton leak in muscle mitochondria from WT and UCP3-/- mice. Following a measure of state 3 respiration, mitochondria were sequentially treated with oligomycin (Oligo; 2.5 μg/mL), carboxyatractyloside (CAT; 5 μM), and antimycin A (4 μM). OCR values were corrected for respiration not associated with the electron transport chain (antimycin A)Student t-test, n=4, mean±SEM.

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