1. Oligonucleotide sequences and PCR conditions used for sequencing Voso et al, Supplementary Figure 1

2. Exemplary sequencing reactions for all genes studied Voso et al, Supplementary Figure 2

3. Time to Leukemic evolution SRSF2-mutated SRSF2-wild type Probability (%) p=0.04 Months from MPN diagnosis Voso et al, Supplementary Figure 3

4. Characteristics of patients with leukemias secondary to chronic myeloproliferative neoplasms *Of 5 patients with a complex karyotype, 2 had 11q23 abnormalities and 3 had a chromosome 5 and/or 7 deletion Voso et al, Supplementary Table 1

5. Prevalence of mutations in patients with leukemias Secondary to chronic myeloproliferative neoplasms * One patient with a previous PV had synchronous IDH2 R140Q and DNMT3A R882H mutations ** p=0.025 Voso et al, Supplementary Table 2