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RFLP Analysis Lab Assignment for Genetics Course

This lab assignment sheet, designed for BIO 210A, focuses on the Restriction Fragment Length Polymorphism (RFLP) analysis. Students will use a DNA probe to identify Bgl-II restriction sites in normal and mutant genes. The exercise involves examining a 6,800 bp DNA sample for genotype determination (NN, NM, MM) following Southern blotting. Students will explore the implications of point mutations on gene expression and inheritance patterns. This hands-on experience enhances understanding of genetic variation and molecular analysis techniques.

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RFLP Analysis Lab Assignment for Genetics Course

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  1. BIO210A Lab Instructor: E. Schmid, Ph.D. Lab Assignment Sheet I(RFLP Analysis Example A) Student Name Date 6,800 bp (= 100%) DNA probe Gene = Bgl-II restriction sites Normal gene(N- allele) * * = Point mutation Gene Mutant gene(M- allele) DNA(bp) RFLP pattern (after Southern Blotting) Genotype NN NM MM

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