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Blood Chimerism Analysis in Parabiotic Mice

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Study on peripheral blood chimerism in parabiotic mice using flow cytometry. Shows percentage of partner-derived blood leukocytes in different parabiont pairs. Data presented with mean and standard deviation. Relates to previous studies on hematopoietic stem cells plasticity and migration.

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Blood Chimerism Analysis in Parabiotic Mice

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  1. Figure S1 Figure S1 Blood chimerism between parabiosed partners. Parabiotic mice were analyzed for the degree of peripheral blood chimerism by flow cytometry. Data are plotted as the mean (+/- SD) percent young partner-derived (Ly5.2+ or GFP+) blood leukocytes present in the bloodstream of young isochronic or old heterochronic parabionts, or of old partner-derived (Ly5.1+) leukocytes present in the blood of young heterochronic parabionts. Flow cytometry was performed as previously described1,2. 1. Wagers,A.J., Sherwood,R.I., Christensen,J.L. & Weissman,I.L. Little evidence for developmental plasticity of adult hematopoietic stem cells. Science297, 2256-2259 (2002). 2. Wright,D.E., Wagers,A.J., Gulati,A.P., Johnson,F.L. & Weissman,I.L. Physiological migration of hematopoietic stem and progenitor cells. Science294, 1933-1936 (2001).

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