1 / 7

TPL2 Inhibition Regulates pERK1/2 Signaling and ERK2 Activity

Explore the impact of TPL2 inhibition on TNF-induced pERK1/2 signaling and ERK2 function. Investigate the role of TPL2 in mediating GST-MEK phosphorylation and MEK activation. Assess effects on UVC-induced p53 expression and response to cis-platin treatment. Examine siTPL2 influence on UVC-induced PARP cleavage and ERK2 signaling modulation.

freddiedavis
Télécharger la présentation

TPL2 Inhibition Regulates pERK1/2 Signaling and ERK2 Activity

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Figure S1

  2. DMSO TPL2 inh TNF 0’ 15’ 0’ 15’ 49 pERK1/2 49 ERK2 Figure S2

  3. IVK MT-ev + - MT-TPL2 - + GST-MEK + + MEK pSer217/221 MEK (GST) TPL2 Figure S3 82 82 64

  4. - - + - + UVC (8h): MG132: Figure S4 64 TPL2 + + - 49 p53 β-actin 49

  5. Α. TPL2: +/+ -/- UVC (hrs): 0 3 5 8 0 3 5 8 p53 β-actin 49 49 B. TPL2 +/+ TPL2 -/- cis-platin (hrs): 0 8 0 8 p53 49 49 ERK2 Figure S5

  6. Α. 48h 24h 48h 24h n.s. n.s. siLuc: siTPL2: UVC : + + + - - - - + - - - - + + + + - - + + + + - - B. siLuc siTPL2 siLuc: + - siTPL2: UVC (hrs): 48 48 - + siLuc siTPL2 20J/m2 UVC: - 48h - 48h C. PARP 82 PARP cleaved 49 ERK2 Figure S6

  7. Np Nu UVC (8h): - + - + pT199 NPM 37 UBF 82 Lamin B 82 Figure S7

More Related